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Series GSE48175 Query DataSets for GSE48175
Status Public on Mar 05, 2014
Title Identification of Myc and Max genomic targets in mouse embryonic stem cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Myc is a master transcription factor that has been demonstrated to be required for embryonic stem cell (ESC) pluripotency, self-renewal, and inhibition of differentiation. Although recent works identified several Myc-targets in ESC the list of Myc binding sites is largely incomplete due to the low sensitivity and specificity of the antibodies available so far. To systematically identify Myc binding sites in mouse ESCs here we used a stringent streptavidin based genome-wide chromatin immunoprecipitation (ChIP-Seq) of a biotin-tagged Myc (Bio-Myc) as well as a ChIP-Seq of the Myc partner Max. This analysis identified 4273 Myc binding sites of which more than 85% co-occupied by Max, overlap with H3K4me3 positive promoters and active enhancers of transcriptional regulators, chromatin modifiers, and genes involved in stem cell self-renewing. The new sites identified were validated experimentally. This study provides a new Myc and Max binding reference in mouse ESCs.
 
Overall design ChIP-seq of bio-Myc and Max in E14 and respective controls; Bio-Myc ChIP-seq was performed in a stable clone of E14 mouse embryonic stem cell expressing Biotin-tagged Myc; Mock, Max and IgG were performed in parental wt E14 mESC.
Web link http://epigenetics.hugef-research.org/data.php
 
Contributor(s) Neri F, Oliviero S
Citation(s) 24586446
Submission date Jun 20, 2013
Last update date Dec 09, 2016
Contact name Francesco Neri
E-mail francesco.neri@hugef-torino.org
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
 
Platforms (1)
GPL16173 Illumina HiScanSQ (Mus musculus)
Samples (4)
GSM1171648 BioMyc_ChIPSeq
GSM1171649 BioMock_ChIPSeq
GSM1171650 Max_ChIPSeq
Relations
BioProject PRJNA209093
SRA SRP026217

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE48175_RAW.tar 240.0 Kb (http)(custom) TAR (of BED)
Processed data provided as supplementary file
Raw data are available in SRA

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