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Series GSE48125 Query DataSets for GSE48125
Status Public on Aug 21, 2013
Title Neonatal antibotic prophylaxis modulates intestinal immunity and prevents necrotizing enterocolitis in preterm neonates
Organism Sus scrofa
Experiment type Expression profiling by array
Summary Caesarean-delivered preterm pigs were fed 3 d of parenteral nutrition followed by 2 d of enteral formula feeding. Antibiotics (n=11) or control saline (n=13) were given twice daily from birth to tissue collection at d 5. NEC-lesions and intestinal structure, function, microbiology and immunity markers were recorded.
We used Affymetrix microarrays to investigate gene expression in intestinal tissues of preterm piglets treated with antibiotics or control saline.
Overall design Twenty-four preterm piglets were delivered by caesarean section on day 105 of gestation from two healthy sows. All piglets were initially provided with parenteral nutrition via a vascular catheter, combined with small amounts of minimal enteral nutrition. On day three, all parenteral nutrition was stopped and total enteral nutrition was given through an oro-gastric feeding tube. Piglets were allocated into controls ( n=13) and an intervention group receiving oral and systemic broad-spectrum antibiotics ( n=11). To assure high systemic and intra luminal MIC values antibiotics were given both orally and intramuscularly. All antibiotics were given directly after feeding with an oral bolus and control pigs were given corresponding amounts of saline. On day five, all piglets were euthanized, and small intestinal tissue collected.
Contributor(s) Kelly D, Mulder I
Citation(s) 24157972
Submission date Jun 19, 2013
Last update date Feb 04, 2014
Contact name Denise Kelly
Phone +44 1224 716648
Organization name University of Aberdeen
Lab Gut Immunology Group
Street address Institute of Medical Sciences
City Aberdeen
ZIP/Postal code AB25 2ZD
Country United Kingdom
Platforms (1)
GPL3533 [Porcine] Affymetrix Porcine Genome Array
Samples (16)
GSM1169044 Antibiotics-proximal 86
GSM1169045 Untreated-Proximal 88
GSM1169046 Untreated-Proximal 89
BioProject PRJNA208962

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Supplementary file Size Download File type/resource
GSE48125_RAW.tar 31.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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