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| Status |
Public on Sep 19, 2013 |
| Title |
Temperature-dependent alternative splicing of FLM controls flowering in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
How plants control the transition to flowering in response to ambient temperature is only beginning to be understood. In Arabidopsis thaliana, the MADS-box transcription factor genes FLOWERING LOCUS M (FLM) and SHORT VEGETATIVE PHASE (SVP) have key roles in this process. FLM is subject to temperature-dependent alternative splicing, producing two splice variants, FLM-β and FLM-δ, which compete for interaction with the floral repressor SVP. The SVP/FLM-β complex is predominately formed at low temperatures and prevents precocious flowering. In contrast, the competing SVP FLM-δ complex is impaired in DNA binding and acts as a dominant negative activator of flowering at higher temperatures. Our results demonstrate the importance of temperature-dependent alternative splicing in modulating the timing of the floral transition in response to environmental change.
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| Overall design |
ChIP-seq A. thaliana FLM (3 replicates for gFLM and 2 replicates for FLM splice variants)
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| Contributor(s) |
Posé D, Verhage L, Ott F, Yant L, Mathieu J, Angenent GC, Immink RG, Schmid M |
| Citation(s) |
24067612 |
| |
| Submission date |
Jun 19, 2013 |
| Last update date |
Sep 04, 2019 |
| Contact name |
Felix Ott |
| Organization name |
Max Planck Institute for Developmental Biology
|
| Street address |
Spemannstr. 35-39
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| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL11221 |
Illumina Genome Analyzer IIx (Arabidopsis thaliana) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA208886 |
| SRA |
SRP026163 |
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