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| Status |
Public on Jun 18, 2013 |
| Title |
Transcriptome analysis of the early response to nickel (Ni) in Acidiphilium sp. PM |
| Organism |
Acidiphilium sp. PM |
| Experiment type |
Expression profiling by array
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| Summary |
To study the mechanisms of Ni resistance in the metal resistant Acidiphilium sp. PM, the transcriptome of Acidiphilium sp. PM was studied 5min and 30 min after the addition of 10mM Ni and compared to the transcriptome in untreated cells.
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| Overall design |
Two-condition hybridization experiments: untreated cells vs cells treated with 10mM Ni (for either 5 or 30 minutes). Eighteen 100ml-cultures: 6 untreated control cultures (not exposed to Ni), 6 cultures exposed to 10mM Ni for 5min, and 6 cultures exposed to 10mM Ni for 30 min. Cultures were independently grown and harvested. Gene expression levels of one untreated replicate were compared separately with one 5min-exposed replicate and with one 30min-exposed replicate (yielding two microarray data sets. One for each hybridization).
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| Contributor(s) |
San Martin-Uriz P, Alcolea PJ, Moreno-Paz M, Gomez MJ, Amils R |
| Citation missing |
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| |
| Submission date |
Jun 17, 2013 |
| Last update date |
Jun 20, 2013 |
| Contact name |
Patxi San Martin-Uriz |
| E-mail(s) |
sanmartinup@cab.inta-csic.es
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| Organization name |
Instituto Nacional de Tecnica Aeroespacial
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| Department |
Centro de Astrobiologia
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| Street address |
Ctra. de Ajalvir, km. 4
|
| City |
Torrejon de Ardoz |
| State/province |
Madrid |
| ZIP/Postal code |
28850 |
| Country |
Spain |
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| Platforms (1) |
| GPL17306 |
Centro de Astrobiologia_Acidiphilium sp. PM shotgun genomic DNA microarray_17184 spots_v1.0 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA208748 |
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