|
| Status |
Public on Jun 18, 2013 |
| Title |
Gene expression in IRfl/fl Cre- and IRfl/fl Cre+ mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The floxed insulin receptor was specifically targetted for deletion in the mammary gland using a mouse strain bearing Cre recombinase under the BLG promoter.
Data analysis: Genespring was used for data analysis
All entities (28853) were filtered on variance between samples, eliminating from further analysis of genes whose coefficient of variation was greater than 50%.
The remaining 2239 genes were filtered on expression levels between 20 and 20,000 intensity units
An unpaired T-test was used to find genes that differed significantly (P<0.05) between experimentals and controls
The remaining 2014 genes were filtered for a 1.1 absolute fold change between experimentals and controls.
Of the remaining expression of 890 genes was down in the Cre+ mice and expression was up for 457 genes in the Cre+ mice.
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| Overall design |
Mouse strains containing a floxed insulin receptor (IRfl/fl) were crossed with a mouse expressing Cre recombinase from a BLG promoter
RNA was applied to Affymetrix MoGene_1_0-st-v1 chips
Three replicates from each strain were used for analysis.
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| |
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| Contributor(s) |
Neville MC, Webb P, Ramanathan P |
| Citation(s) |
23982156 |
| |
| Submission date |
Jun 17, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Margaret C Neville |
| E-mail(s) |
peggy.neville@ucdenver.edu
|
| Phone |
970 468 0668
|
| Organization name |
UCDenver
|
| Department |
OB/GYN
|
| Street address |
712 Eveningstar Rd
|
| City |
Silverthorne |
| State/province |
CO |
| ZIP/Postal code |
80498 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
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| Relations |
| BioProject |
PRJNA208629 |
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