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Series GSE47752 Query DataSets for GSE47752
Status Public on Jun 08, 2013
Title Transcriptional profiling of dentate granule cells in 4 rat epilepsy models
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Global expression profiling of epileptogenesis has been confounded by variability across laboratories, epilepsy models, tissue sampled and experimental platforms, with the result that very few genes demonstrate consistent expression changes. The present study minimizes these confounds by combining Affymetrix microarray datasets from seven laboratories, using three status epilepticus (SE) models of epilepsy in rats (pilocarpine, kainate, self-sustained SE or SSSE) and the rat kindling model. Total RNA was harvested from laser-captured dentate granule cells from 6 rats at three times during the early-to-mid latent phase that precedes epilepsy symptoms in the SE models (1, 3 and 10 days after SE), or 24 hr after the first stage 2, stage 4 and stage 5 seizure in the kindling model. Each epilepsy model was studied in two independent laboratories except SSSE. The initial goals of this study were to a) identify model-independent transcriptional changes in dentate granule cells that could point to novel intervention targets for epileptogenesis, b) characterize the basal transcriptional profile of dentate granule cells, and c) identify genes that have highly variable expression.
Overall design Each experimental group consists of 6 rats (biological replicates) from one laboratory at a single time point, except for the SSSE group (6 at day 1 after SSSE, 5 controls and at day 3 after SSSE, 4 at day 10). Thus granule cells were harvested from 164 rats.
Contributor(s) Dingledine R, Coulter D, Nadler JV, Wadman W, Wasterlain C, McNamara JO, Rogawski M, Sloviter R
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Submission date Jun 07, 2013
Last update date Jul 31, 2017
Contact name Winnie Liang
Organization name Translational Genomics
Street address 445 N. Fifth Street
City Phoenix
State/province AZ
ZIP/Postal code 85012
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (172)
GSM1155785 granule cell-SSSE-Wasterlain-control-rat 1
GSM1155786 granule cell-kindling-McNamara-control-rat 1
GSM1155787 granule cell-kindling-Rogawski-control-rat 1
BioProject PRJNA207573

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Supplementary file Size Download File type/resource
GSE47752_RAW.tar 516.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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