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Series GSE4744 Query DataSets for GSE4744
Status Public on May 05, 2006
Title Transcriptional profiling of stress-response in cultured porcine islets
Organism Sus scrofa
Experiment type Expression profiling by array
Summary An increasing demand for cell-based diabetes therapy could be met through xenotransplantation of adult porcine islets. Use of islet xenotranplantation on a large scale would require rigorous safety and quality control measures to maximize transplant success. Development of molecular tools to monitor porcine islet cellular responses to ischemic, osmotic, mechanical and oxidative stresses during islet cell processing and post-isolation culturing would aid the rational design of cytoprotective strategies aimed at improving transplant outcomes. In addition, gene expression signatures informative for islet quality could serve as an adjunct to physiological testing to establish the suitability of islet products for transplantation.
Keywords: stress response
Overall design Nine adult Landrace sows (2-3.5 y old, 249±27 kg) were sacrificed, the pancreases were dissected, and islet cells isolated as previously described [8]. Islet preparation purity was assessed by light microscopy after staining with diphenylthiocarbazone and ranged between 90-95% for the preparations used. Islet yield, based on islet equivalents (IEQ, number of islets standardized to 150 µm diameter), was estimated at 1930±520 per gram of pancreas tissue.Profiles of islet cells cultured under standard conditions were compared to islet cells cultured under stress conditions with elevated glucose (16.7 mM) or addition of inflammatory cytokines (IL-1beta, TNF-alpha, and IFN-gamma), or both, for 48 hours.
Contributor(s) Dvorak C, Hårdstedt M, Xie H, Wang M, Papas k, Juneja B, Murtaugh M, Fahrenkrug S
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Submission date May 01, 2006
Last update date May 28, 2013
Contact name Bhupinder Juneja
Organization name University Of Minnesota
Street address 1988 Fitch Avenue S
City Minneapolis
State/province MN
ZIP/Postal code 55414
Country USA
Platforms (1)
GPL3707 Pig_Array_Ready Oligo set v1.0
Samples (30)
GSM106784 UMN/DiabetesInstitute/FahrenkrugLab-SS-Q02-14688-V1.0
GSM106785 UMN/DiabetesInstitute/FahrenkrugLab-SS-QO3-14688-V1.0
GSM106786 UMN/DiabetesInstitute/FahrenkrugLab-SS-QO4-14688-V1.0
BioProject PRJNA95687

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