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Series GSE4733 Query DataSets for GSE4733
Status Public on Apr 27, 2006
Title Transcriptional regulators of stamen development in Arabidopsis identified by transcriptional profiling
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in jasmonate synthesis, such as opr3, are male-sterile but become fertile when jasmonate is applied to developing flower buds. We have used ATH1 oligonucleotide arrays to follow gene expression in opr3 stamens for 22 hours following jasmonate treatment. In these experiments, a total of 821 genes were specifically induced by jasmonate and 480 repressed. Comparisons with data from previous studies indicate that these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen. Bioinformatics tools allowed us to associate many of the induced genes with metabolic pathways that are likely up-regulated during jasmonate-induced maturation. Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes. Extensive additional analysis of our dataset identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate. Two of these transcription factors, MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins. A myb21 mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility. A myb24 mutant was phenotypically wild type, but production of a myb21 myb24 double mutant indicated that introduction of the myb24 mutation exacerbated all three aspects of the myb21 phenotype. Exogenous jasmonate could not restore fertility to myb21 or myb21 myb24 mutant plants. Together with the data from transcriptional profiling, these results indicate that MYB21 and MYB24 are induced by jasmonate and mediate important aspects of the jasmonate response during stamen development.
Keywords: Time course analysis
Overall design Three replicates for opr3 at each time point of 0 hour, 30 minutes, 2 hours, 8 hours and 22 hourss of either JA or OPDA treatment. One replicate of wild-type stamens
Contributor(s) Mandaokar A, Thines B, Shin B, Lange M, Choi G, Koo Y, Yoo Y, Choi Y, Choi G, Browse J
Citation(s) 16805732
Submission date Apr 27, 2006
Last update date Aug 28, 2018
Contact name Ajin D Mandaokar
Phone 509-335-2337
Fax 509-335-7643
Organization name Washington State University
Department Institute of Biological Chemistry
Lab Browse lab
Street address 447, Clark hall
City Pullman
State/province WA
ZIP/Postal code 99164-6340
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (27)
GSM106825 opr3_0 hr_Rep1
GSM106827 opr3_0 hr_Rep2
GSM106828 opr3_0 hr_Rep3
Affiliated with GSE69995
BioProject PRJNA95667

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Supplementary file Size Download File type/resource
GSE4733_RAW.tar 91.4 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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