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Series GSE47190 Query DataSets for GSE47190
Status Public on Oct 09, 2013
Title ChIP-seq of MYST acetyltransferases
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Histone acetyltransferases (HAT) assemble into multisubunit complexes in order to target distinct lysine residues on nucleosomal histones. Here, we characterize native HAT complexes assembled by the BRPF-family of scaffold proteins. Their PHD-ZnKnuckle-PHD domain is essential for binding chromatin and restricted to unmethylated H3K4, a specificity that is reversed by the associated ING subunit. Native BRPF1 complexes can contain either MOZ/MORF or HBO1 as catalytic acetyltransferase subunit. Interestingly, while the previously reported HBO1 complexes containing JADE scaffold proteins target histone H4, the HBO1-BRPF1 complex acetylates only H3 in chromatin. We mapped a small region at the N-terminus of scaffold proteins responsible for histone tail selection on chromatin. Thus, alternate choice of subunits associated with HBO1 can switch its specificity from H4 to H3 tails, highlighting a crucial new role of associated subunits within HAT complexes, previously thought to be intrinsic to the catalytic subunit.
Overall design Genome-wide mapping of MYST acetyltransferases subunits and H3K4me3 histone mark in RKO cells.
Contributor(s) Lalonde M, Paquet ER, Côté J
Citation(s) 24065767
Submission date May 22, 2013
Last update date May 15, 2019
Contact name Marie-Eve Lalonde
Organization name CHUQ
Street address 9 McMahon street
City Quebec city
State/province Qc
ZIP/Postal code G1R 2J6
Country Canada
Platforms (2)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM1146447 ING2
GSM1146448 ING5
GSM1146449 Brpf2
BioProject PRJNA205078
SRA SRP023013

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Supplementary file Size Download File type/resource
GSE47190_RAW.tar 3.2 Gb (http)(custom) TAR (of BED, BW)
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Processed data provided as supplementary file
Raw data are available in SRA

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