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Series GSE47114 Query DataSets for GSE47114
Status Public on Jul 25, 2013
Title RNA-Guided Human Gene Activation by Cas9/CRISPR-Based Engineered Transcription Factors
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Synthetic transcription factors can be applied in many areas of biotechnology, medicine, and basic research.  In contrast to current methods based on engineering new DNA-binding proteins, we show that Cas9 fused to a transcriptional activation domain can be targeted by combinations of guide RNA molecules to induce the expression of endogenous human genes. This simple approach for targeted gene activation circumvents the need for engineering new proteins and will enable widespread synthetic gene regulation.
Overall design HEK293T cells were transfected with plasmid expressing Cas9-VP64 fusion protein and a guide RNA. As a control, empty guide RNA was transfected. Gene expression was then measured using mRNA-seq, and differential expression calculated using DESeq. All experiments were performed in biological duplicates or triplicates.
Contributor(s) Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Ousterout DG, Kabadi AM, Thakore PI, Polstein LR, Glass KA, Leong KW, Guilak F, Crawford GE, Reddy TE, Gersbach CA
Citation(s) 23892895
Submission date May 20, 2013
Last update date Mar 20, 2019
Contact name Timothy E Reddy
Organization name Duke University
Department Department of Biostatistics & Bioinformatics
Lab ReddyLab
Street address 2347 CIEMAS, 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (7)
GSM1145136 Control treated, Rep 1
GSM1145137 Control treated, Rep 2
GSM1145138 IL1RN CRISPRs, Rep 1
BioProject PRJNA203652
SRA SRP022913

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Supplementary file Size Download File type/resource 510.5 Kb (ftp)(http) TAB
Raw data are available in SRA
Processed data is available on Series record

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