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| Status |
Public on Jul 15, 2013 |
| Title |
In Vivo Endothelial Cell Heterogeneity |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Endothelial cells from nine steady state tissues and two regenerating tissues (bone marrow and liver) were intravitally labeld, isolated via flow sorting, and immediately processed for RNA extraction.
When of sufficient quality, the RNA was amplified and hybridized. For comparison, Human Emybryonic Stem Cell-derived Endothelial cells (hESC-ECs) were differentiated and isolated based on similarities to the adult mouse counterparts.
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| |
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| Overall design |
Endothelial cells were labeled via intravitally labeling of the vascular bed 8 minutes prior to sacrifice with minimally three markers to identify endothelial cells followed by flow sorting.
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| Contributor(s) |
Nolan DJ, Rafii S |
| Citation(s) |
23871589 |
| |
| Submission date |
May 17, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Daniel J Nolan |
| E-mail(s) |
dnolan@angiocrinebio.com
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Genetic Medicine
|
| Lab |
Rafii
|
| Street address |
1300 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (57)
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| Relations |
| BioProject |
PRJNA203650 |
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