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Series GSE46719 Query DataSets for GSE46719
Status Public on May 09, 2013
Title Murine splenic macrophage Cells: Control mice vs. Schistosoma japonicum-infected mice
Organism Mus musculus
Experiment type Expression profiling by array
Summary Macrophages initiate, modulate, and also serve as final effector cells in immune responses during course of schistosomal infections. Presently, we discussed the roles of the gene expression profile and functional changes of macrophages in immune responses against the Schistosoma japonicum by microarray experiments. Hierarchical clustering analysis demonstrated that a significant switch in gene transformation associated with a type-1 response and linked with a type-2 cytokine phenotype occurs between 4.5 and 8 weeks post-infection. Moreover, the gene profiles at 3 later time-points following egg challenge were similar in complexity and magnitude. These data also showed that there are mostly inhibition in gene expression related TLR, IFN, MHC and TNFrsf at the switch between 4.5 and 8 weeks post-infection, It is suggested that these immunomodulatory genes may be down-regulated in resistance against S. japonicum eggs and granuloma pathology. The induction of alternatively activated macrophage was important for dampening the inflammation in hepatic granulomas and contributing to a decrease in cytotoxicity. The genes expressions involved in repair/remodeling during liver fibrosis were also observed after eggs production. Understanding these immune mechanisms related to parasite resistance, pathology, and growth with regard to the disease will be helpful in further studies on S. japonicum.
 
Overall design Two-condition experiment, Control mice vs. Schistosoma japonicum-infected mice. replicates: 2 infected replicates. Different post-infection weeks
 
Contributor(s) Cheng P, Lee K
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Submission date May 08, 2013
Last update date May 11, 2013
Contact name Po-Ching Cheng
E-mail(s) bonjovi@tmu.edu.tw
Organization name Taipei Medical University
Department Department of Parasitology, College of Medicine
Street address 250 Wuxing St,
City Taipei
ZIP/Postal code 11031
Country Taiwan
 
Platforms (1)
GPL17135 Agilent-015720 Mus musculus 15K
Samples (10)
GSM1136099 Schistosoma japonicum post-infection at 1 week Replicate 1
GSM1136100 Schistosoma japonicum post-infection at 1 week Replicate 2
GSM1136101 Schistosoma japonicum post-infection at 4.5 week Replicate 1
Relations
BioProject PRJNA202098

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46719_RAW.tar 47.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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