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Series GSE46488 Query DataSets for GSE46488
Status Public on Dec 02, 2013
Title Evolutionary conservation of the eumetazoan gene regulatory landscape
Organism Nematostella vectensis
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Bilaterian animals differ from other metazoans in their apparent bilateral symmetry and the development of a third germ layer. Both might have facilitated the evolution of the diverse and complex bilaterian body plans. The first cnidarian genome sequence revealed that despite their morphological simplicity, this sister group to all bilaterians shares an immense genomic complexity with vertebrates. This suggested that it might have been the complexity of gene regulation which increased during the evolution of bilaterians. We compared the gene regulatory landscape of cnidarians and bilaterians. To this end we generated the first genome-wide prediction of gene regulatory elements and profiled five epigenetic marks in a non-bilaterian animal, the cnidarian Nematostella vectensis. We found that the location of chromatin modifications relative to genes and distal enhancers is conserved among eumetazoans. Surprisingly, the genomic landscape of gene regulatory elements is highly similar between Nematostella and bilaterian model organisms. This suggests that complex regulation of developmental gene expression evolved in eumetazoans without a major increase in complexity in bilaterians.
 
Overall design ChIP-seq of p300, RNA Pol2, and five histone modifications in Nematostella vectensis.
 
Contributor(s) Schwaiger M, Schönauer A, Rendeiro AF, Pribitzer C, Schauer A, Gilles A, Schinko J, Renfer E, Fredman D, Technau U
Citation(s) 24642862
Submission date Apr 29, 2013
Last update date Mar 31, 2014
Contact name Michaela Schwaiger
E-mail michaela.schwaiger@univie.ac.at
Organization name University of Vienna
Lab Technau
Street address Althanstr. 14
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platforms (2)
GPL9914 Illumina Genome Analyzer II (Nematostella vectensis)
GPL17090 Illumina HiSeq 2000 (Nematostella vectensis)
Samples (29)
GSM1131157 H3K27ac_adultfemale
GSM1131158 H3K27ac_gastrula
GSM1131159 H3K27ac_planula
Relations
BioProject PRJNA200689
SRA SRP021895

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46488_RAW.tar 191.3 Mb (http)(custom) TAR (of BED)
Processed data provided as supplementary file
Raw data are available in SRA

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