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Series GSE46166 Query DataSets for GSE46166
Status Public on Jun 30, 2014
Title Insights into the invasiveness of triple negative breast cancer from genome-wide profiling of transcription factor AP-1 (ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Triple negative breast cancer (TNBC) is an aggressive clinical phenotype, and accounts for 15% to 20% of all breast cancers. The molecular determinants of malignant cell behaviors in TNBC remain largely unknown. We find that the AP-1 transcription factor component, Fra-1, is overexpressed in basal-like breast tumors, and its expression level has high prognostic significance. Depletion of Fra-1 or its heterodimeric partner c-Jun inhibits the proliferative and invasive phenotypes in TNBC cells. To gain insights into the transcriptional regulatory networks of AP-1 in TNBC cells, we combine genome-wide ChIP-seq with loss-of-function transcriptome analyses. We observe dysregulation of direct targets of the Fra-1/c-Jun heterodimer involved in cell proliferation, cell adhesion, and cell-cell contact. Intriguingly, we find that AP-1 mediates downregulation of E-cadherin through direct transcriptional induction of ZEB2. This work sheds light on the mechanisms and pathways by which TNBC acquires invasiveness and proliferative propensity.
Overall design Examination of genome-wide binding sites of AP-1 transcription factor components, Fra-1 and c-Jun, in BT549 breast cancer cell line.
Contributor(s) Qiao Y, Dahlman-Wright K, Jonsson P, Williams C, Xu L, Sinha I, Zhao C
Citation(s) 24830720
Submission date Apr 17, 2013
Last update date May 15, 2019
Contact name Chunyan Zhao
Phone 46-8-52481126
Fax 46-8-7745538
Organization name Karolinska Institute
Department Biosciences and Nutrition
Street address Hälsovägen 7-9
City Stockholm
ZIP/Postal code SE-171 77
Country Sweden
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (4)
GSM1125261 Fra1_ChIPSeq
GSM1125262 cJun_ChIPSeq
GSM1125263 IgG_ChIPSeq
This SubSeries is part of SuperSeries:
GSE46441 Insights into the invasiveness of triple negative breast cancer from genome-wide profiling of transcription factor AP-1
BioProject PRJNA197435
SRA SRP021169

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46166_Peaks_cJun_reff_IgG_200.bed.gz 123.4 Kb (ftp)(http) BED
GSE46166_Peaks_cJun_reff_input_200.bed.gz 130.2 Kb (ftp)(http) BED
GSE46166_Peaks_fra1_reff_IgG_200.bed.gz 98.7 Kb (ftp)(http) BED
GSE46166_Peaks_fra1_reff_input_200.bed.gz 107.5 Kb (ftp)(http) BED
GSE46166_RAW.tar 1.1 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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