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Series GSE45852 Query DataSets for GSE45852
Status Public on Jul 21, 2013
Title Tight coordination of protein translation and heat shock factor 1 activation supports the anabolic malignant state [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A unifying characteristic of aggressive cancers is a profound anabolic shift in metabolism to enable sustained proliferation and biomass expansion. The ribosome is centrally situated to sense metabolic states but whether it impacts systems that promote cellular survival is unknown. Here, through integrated chemical-genetic analyses, we find that a dominant transcriptional effect of blocking protein translation in cancer cells is complete inactivation of heat shock factor 1 (HSF1), a multifaceted transcriptional regulator of the heat-shock response and many other cellular processes essential for tumorigenesis. Translational flux through the ribosome reshapes the transcriptional landscape and links the fundamental anabolic processes of protein production and energy metabolism with HSF1 activity. Targeting this link deprives cancer cells of their energy and chaperone armamentarium thereby rendering the malignant phenotype unsustainable.
Overall design We used ChIP-Seq to examine affect of rocaglates and cycloheximide on HSF1 genomic occupancy in MCF7 and M0-91 cancer cells.
Contributor(s) Santagata S, Mendillo ML, Tang Y, Subramanian A, Perley CC, Roche SP, Wong B, Narayan R, Kwon H, Koeva M, Amon A, Golub TR, Porco JA, Whitesell L, Lindquist S
Citation(s) 23869022
Submission date Apr 08, 2013
Last update date May 15, 2019
Contact name Marc Mendillo
Organization name Whitehead Institute for Biomedical Research
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (21)
GSM1116653 MCF7_DMSO_HSF1_R1
GSM1116654 MCF7_DMSO_HSF1_R2
This SubSeries is part of SuperSeries:
GSE45853 Tight coordination of protein translation and heat shock factor 1 activation supports the anabolic malignant state
BioProject PRJNA196591
SRA SRP020615

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Supplementary file Size Download File type/resource
GSE45852_Table_S3.xlsx.gz 6.7 Mb (ftp)(http) XLSX
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