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| Status |
Public on Apr 06, 2013 |
| Title |
Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein |
| Organism |
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 |
| Experiment type |
Protein profiling by protein array
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| Summary |
A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.
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| Overall design |
Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was separated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interactions, rabbit polyclonal IgG to C. jejuni was used, while unspecific binding to the epitope sequence was checked using rabbit polyclonal IgG to Salmonella enterica.
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| Citation(s) |
23734261 |
| Submission date |
Mar 27, 2013 |
| Last update date |
Jul 09, 2013 |
| Contact name |
Sebastian Hoppe |
| E-mail(s) |
sebastian.hoppe@izi-bb.fraunhofer.de
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| Organization name |
Fraunhofer Institute for Cell Therapy and Immunology
|
| Department |
Bioanalytics and Biosensorics
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| Lab |
Molecular Bioengineering
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| Street address |
Am Mühlenberg 13
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| City |
Potsdam |
| ZIP/Postal code |
14476 |
| Country |
Germany |
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| Platforms (1) |
| GPL16889 |
IBMT Campylobacter jejuni alanine scan |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE44717 |
Identification of novel immunodominant proteins from Campylobacter jejuni and the characterization of linear epitopes |
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| Relations |
| BioProject |
PRJNA194602 |
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