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Series GSE45556 Query DataSets for GSE45556
Status Public on Apr 06, 2013
Title Alanine scan of 11 amino acid long novel linear epitope from C. jejuni cj0669 protein
Organism Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Experiment type Protein profiling by protein array
Summary A short sequence of 11 amino acids belonging to the cj0669 protein from Campylobacter jejuni NCTC 11168, which was previously identified as potentially immunogenic, was analyzed via alanine scanning to narrow down the significant amino acid residues within the sequence.
 
Overall design Twelve peptides, one representing the original sequence and eleven peptides with each residue replaced by alanine in turn, were synthesized on microarrays by JPTs Pepstar Technology. For each microarray, nine replicates for each peptide were spotted. The microarray was separated into three incubation chambers by the ProPlate 3-well module (Grace Biolabs) to allow for incubation with different antibodies in parallel. For specific interactions, rabbit polyclonal IgG to C. jejuni was used, while unspecific binding to the epitope sequence was checked using rabbit polyclonal IgG to Salmonella enterica.
 
Citation(s) 23734261
Submission date Mar 27, 2013
Last update date Jul 09, 2013
Contact name Sebastian Hoppe
E-mail(s) sebastian.hoppe@izi-bb.fraunhofer.de
Organization name Fraunhofer Institute for Cell Therapy and Immunology
Department Bioanalytics and Biosensorics
Lab Molecular Bioengineering
Street address Am M├╝hlenberg 13
City Potsdam
ZIP/Postal code 14476
Country Germany
 
Platforms (1)
GPL16889 IBMT Campylobacter jejuni alanine scan
Samples (10)
GSM1109478 alascan, rep1
GSM1109479 alascan, rep2
GSM1109480 alascan, rep3
This SubSeries is part of SuperSeries:
GSE44717 Identification of novel immunodominant proteins from Campylobacter jejuni and the characterization of linear epitopes
Relations
BioProject PRJNA194602

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45556_A10.gpr.gz 79.2 Kb (ftp)(http) GPR
GSE45556_A11.gpr.gz 73.8 Kb (ftp)(http) GPR
GSE45556_A6.gpr.gz 80.5 Kb (ftp)(http) GPR
GSE45556_A7.gpr.gz 80.8 Kb (ftp)(http) GPR
GSE45556_A8.gpr.gz 84.1 Kb (ftp)(http) GPR
GSE45556_RAW.tar 30.0 Kb (http)(custom) TAR
Raw data are available on Series record
Processed data included within Sample table

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