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Series GSE45115 Query DataSets for GSE45115
Status Public on Apr 24, 2014
Title Expression profiling of 376 wildtypes to assess day-to-day variance
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary A collection of budding yeast wild types grown in parallel to 1,484 budding yeast deletion mutants to assess day-to-day variance. Related to series GSE42526 and GSE42527.
 
Overall design Two channel microarrays were used. RNA isolated from a large amount of wt yeast from a single culture was used as a common reference. This common reference was used in one of the channels for each hybridization and used in the statistical analysis to obtain an average expression-profile for each deletion mutant relative to the wt. Two independent cultures were hybridized on two separate microarrays. For the first hybridization the Cy5 (red) labeled cRNA from the deletion mutant is hybridized together with the Cy3 (green) labeled cRNA from the common reference. For the replicate hybridization, the labels are swapped. Each gene is represented twice on the microarray, resulting in four measurements per mutant. Using the Erlenmeyer growth protocol up to five deletion strains were grown on a single day. In the tecan platereader, up to eleven deletion strains could be grown on a single day. Wt cultures were grown parallel to the deletion mutants to assess day-to-day variance.
 
Contributor(s) Kemmeren P, Sameith K, van de Pasch L, Holstege F
Citation(s) 24766815
Submission date Mar 12, 2013
Last update date Apr 30, 2014
Contact name Patrick Kemmeren
Organization name UMC Utrecht
Department Department of Molecular Cancer Research
Lab Holstege Lab
Street address Universiteitsweg 100
City Utrecht
State/province Utrecht
ZIP/Postal code 3584 CG
Country Netherlands
 
Platforms (1)
GPL11232 A-UMCU-Y16k-1.3
Samples (755)
GSM630359 wt-SV013-a
GSM630360 wt-SV013-b
GSM630361 wt-ea011-1-a
Relations
BioProject PRJNA192934

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45115_RAW.tar 497.9 Mb (http)(custom) TAR (of TXT)
GSE45115_protocol_full_description.txt.gz 10.0 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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