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| Status |
Public on Apr 30, 2013 |
| Title |
Gene expression profiling of pancreatic cancer cell line BxPC-3 induced by PAR-2 AP |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Protease-activated receptor-2 (PAR-2), a G protein-coupled receptor activated by trypsin and coagulant factors, plays broad spectrum of physiological and pathological roles especially in cancer development. In this study, we used PAR-2 activating peptide to mimic the action of trypsin to trigger PAR-2 signaling pathway and effects of PAR-2 activation on gene expression in human pancreatic cancer cell line BxPC-3 investigated by microarray analysis.
Through DAVID bioinformatic resources, we observed that activated PAR-2-mediated genes are summarized to two different pathways, renal cell carcinoma and NFkB pathway. In renal cell carcinoma pathway, activated PAR-2 dysregulated hypoxia-inducible factors and its target genes, including glucose transporter 1 (GLUT1), transforming growth factor-b (TGF-b) and vascular endothelial growth factor-A (VEGF-A). In addition, activated PAR-2 induced MAPK signaling and transcriptional factors, such as JUN, MAP2K1 and ETS1. The regulation of these genes by PAR-2 assumed that PAR-2 signaling was associated with cancer progression.
On the other hand, activated PAR-2 upregulated interleukin-1b (IL-1b) and toll-like receptor 4 (TLR4) related with NFkB activation, which indicated that PAR-2 signaling may cause cancer-related inflammation. In conclusion, PAR-2 may be a factor to regulate cancer progression and inflammation.
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| Overall design |
Two-condition experiment, control cells vs PAR-2 AP-treated cells.
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| Contributor(s) |
Chang L, Pan S, Lai C, Tsai A, Teng C |
| Citation(s) |
23764046 |
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| Submission date |
Mar 04, 2013 |
| Last update date |
Jan 20, 2016 |
| Contact name |
Che-Ming Teng |
| E-mail(s) |
cmteng@ntu.edu.tw
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| Phone |
+886-2-23123456
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| Fax |
+886-2-23221742
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| Organization name |
National Taiwan University
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| Department |
Pharmacology
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| Street address |
No.1, Jen-Ai Road
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| City |
Taipei |
| ZIP/Postal code |
100 |
| Country |
Taiwan |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA192255 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE44827_RAW.tar |
5.6 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE44827_normalized.txt.gz |
614.7 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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