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Series GSE4470 Query DataSets for GSE4470
Status Public on Mar 15, 2006
Title Heart transplant biopsies-second test set
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MOLECULAR PROFILING IMPROVES DIAGNOSES OF REJECTION AND INFECTION IN TRANSPLANTED ORGANS.
The monitoring of transplanted hearts is currently based on histological evaluation of endomyocardial biopsies, a method that is fairly insensitive and that does not always accurately discriminate between rejection and infection in the heart. Accurate diagnosis of rejection and infection is absolutely crucial, however, as the respective treatments are completely different.
Using microarrays we analyzed gene expression in 76 cardiac biopsies from 40 heart recipients undergoing rejection, no rejection, or T. cruzi infection. We found a set of 98 genes whose expression patterns were typical of acute rejection, and 87 genes that discriminated between rejection and T. cruzi infection. These sets revealed acute rejection episodes up to two weeks earlier, and trypanosome infection up to two months earlier than did histological evaluation. When applied to raw data from other institutions, the two sets of predictive genes were also able to accurately pinpoint acute rejection of lung and kidney transplants, as well as bacterial infections in kidneys. In addition to their usefulness as diagnostic tools, the data suggest that there are similarities in the biology of the processes involved in rejection of different grafts and also in the tissue responses to pathogens as diverse as bacteria and protozoa.
Keywords: disease state analysis
 
Overall design This series includes 24 samples that were used to validate acute rejection profile descovered previously (GSE2596). This set of hybridizations was done using different from GSE2596 reference RNA (Cy5-labeled). Therefore, Cy3/Cy5 log-ratios could not be compared between these two series. There are 27 arrays in this series because 3 samples were done in duplicates that can be identified by first numbers in the title of the sample.
 
Contributor(s) Morgun A, Shulzhenko N, Perez-Diez A, Diniz RV, Sanson GF, Almeida DR, Matzinger P, Gerbase-DeLima M
Citation(s) 16794196
Submission date Mar 14, 2006
Last update date Jan 18, 2013
Contact name Natalia Shulzhenko
E-mail(s) anemorgun@hotmail.com, shulzhenkon@niaid.nih.gov
Phone 1 301 4355817
Fax 1 301 4964286
Organization name NIH
Department NIAID
Lab Ghost Lab, LCMI
Street address 9000 Rockville Pike, Building 4, room 111
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL1053 NIAID - Hsab
Samples (27)
GSM100333 1137 (R) Hsac-02-28
GSM100402 1068 (R) Hsac-02-29
GSM100404 1094 (N) Hsac-02-30
Relations
BioProject PRJNA94541

Clinical data header descriptions
GEO Accession
GEO Title
aRNA
replicate array
"Patient&sample ID (letter=patient ID, number=days after Tx):"
Sex:
Basic disease:
Days after Tx:
Histology:
Clinical history notes:
Final Clinical diagnosis (R=rejection; Ch=Chagas disease reactivation; N=non-rejection&non-Chagas):

Data table
GEO Accession GEO Title aRNA replicate array "Patient&sample ID (letter=patient ID, number=days after Tx):" Sex: Basic disease: Days after Tx: Histology: Clinical history notes: Final Clinical diagnosis (R=rejection; Ch=Chagas disease reactivation; N=non-rejection&non-Chagas):
GSM100402 1068 (R) Hsac-02-29 1068 Y AG243 male Chagas cardiomyopathy 243 3A "presented clinical signs of rejection, change of immunosuppresssion" R
GSM100434 1068 (R) Hsac-02-71 1068 Y AG243 male Chagas cardiomyopathy 243 3A "presented clinical signs of rejection, change of immunosuppresssion" R
GSM100419 1128 (R) Hsac-02-51 1128 N AK74 male Chagas cardiomyopathy 74 3A R
GSM100333 1137 (R) Hsac-02-28 1137 N AN7 male dilated cardiomyopathy 7 3A anti-rejection pulse treatment R
GSM100429 1140 (R) Hsac-02-66 1140 N AN14 male dilated cardiomyopathy 14 3A R
GSM100437 1152 (R) Hsac-02-75 1152 N AM14 female Chagas cardiomyopathy 14 3A anti-rejection pulse treatment R
GSM100408 1156 (R) Hsac-02-32 1156 N AQ224 male Chagas cardiomyopathy 224 3A R
GSM100420 1159 (R) Hsac-02-56 1159 N AM26 female Chagas cardiomyopathy 26 3A R
GSM100431 1162 (R) Hsac-02-68 1162 N AM41 female Chagas cardiomyopathy 41 3A R
GSM100432 1165 (R) Hsac-02-69 1165 N AN24 male dilated cardiomyopathy 24 3A R
GSM100422 1168 (R) Hsac-02-57 1168 N AH8y male Chagas cardiomyopathy 8y 3A cardiac dysfunction R
GSM100424 1171 (R) Hsac-02-58 1171 N AO6y female congenital cardiomyopathy 6y 3B anti-rejection pulse treatment R
GSM100416 1119 (pre-R) Hsac-02-43 1119 Y AQ68 male Chagas cardiomyopathy 68 0 15 days pre-Rejection Pre-R
GSM100418 1119 (pre-R) Hsac-02-46 1119 Y AQ68 male Chagas cardiomyopathy 68 0 15 days pre-Rejection Pre-R
GSM100410 1077 (N) Hsac-02-35 1077 Y AP10 male Chagas cardiomyopathy 10 0 N
GSM100435 1077 (N) Hsac-02-73 1077 Y AP10 male Chagas cardiomyopathy 10 0 N
GSM100428 1079 (N) Hsac-02-65 1079 N AP17 male Chagas cardiomyopathy 17 0 N
GSM100404 1094 (N) Hsac-02-30 1094 N AI83 male dilated cardiomyopathy 83 0 N
GSM100412 1103 (N) Hsac-02-40 1103 N AQ9 male Chagas cardiomyopathy 9 0 N
GSM100413 1106 (N) Hsac-02-41 1106 N AQ18 male Chagas cardiomyopathy 18 0 N

Total number of rows: 50

Table truncated, full table size 2 Kbytes.




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