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| Status |
Public on Dec 02, 2013 |
| Title |
Next generation sequencing facilitates quantitative analysis of Caenorhabditis elegans N2 wild type, emr-1(gk119), lem-2(tm1582) and emr-1(RNAi) lem-2(tm1582) transcriptomes. |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Laminopathies are caused by mutations in components of the nuclear envelope (NE). While most NE components are widely expressed, laminopathies affect only a subset of tissues. However, the understanding of the molecular mechanisms that explain this phenomenon is still elusive. Here we have performed RNA-Seq analysis in adult C. elegans nematodes comparing gene expression in wild type and single and double mutants of two components of the NE, EMR-1 and LEM-2. Our data confirm that EMR-1 and LEM-2 facilitate gene repression and that both proteins control the expression of mainly muscle and neuronal genes.
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| Overall design |
mRNA profiles of wild type, emr-1(gk119), lem-2(tm1582) and emr-1(RNAi) lem-2(tm1582) young adult worms were generated by deep sequencing, in triplicate for the wild type and duplicates for the other backgrounds, using Illumina GAIIx.
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| Contributor(s) |
González-Aguilera C, Askjaer P |
| Citation(s) |
24490688 |
| |
| Submission date |
Feb 26, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Askjaer |
| E-mail(s) |
pask@upo.es
|
| Phone |
+34 954 348 396
|
| Organization name |
Centro Andaluz de Biología del Desarrollo
|
| Street address |
UPO Carretera de Utrera km 1
|
| City |
Seville |
| ZIP/Postal code |
41013 |
| Country |
Spain |
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| Platforms (1) |
| GPL13776 |
Illumina Genome Analyzer IIx (Caenorhabditis elegans) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA190895 |
| SRA |
SRP018867 |
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