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Series GSE44643 Query DataSets for GSE44643
Status Public on Sep 27, 2013
Title Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells [Expression array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The de novo DNA methyltransferase 3-like (Dnmt3L) is a catalytically inactive DNA methylase that has been previously shown to cooperate with Dnmt3a and Dnmt3b to methylate DNA. Dnmt3L is highly expressed in mouse embryonic stem cells (ESC) but its function in these cells is unknown. We here report that Dnmt3L is required for the differentiation of ESC into primordial germ cells (PGC) through activation of the homeotic gene Rhox5. By genome-wide analysis we found that Dnmt3L is a positive regulator of methylation at gene bodies of housekeeping genes and a negative regulator of methylation at promoters of bivalent genes. We demonstrate that Dnmt3L interacts with the Polycomb PRC2 complex in competition with the DNA methyl transferases Dnmt3a and Dnmt3b to maintain low the methylation level at H3H27me3 regions. Thus in ESC, Dnmt3L counteracts the activity of de novo DNA methylases to keep low the level of DNA methylation at developmental gene promoters.
Overall design Total RNA extracted from shGFP or shDnmt3L (three different) embryonic stem cells. A duplicate was performed for each point. Cells were transfected with shRNA and selected with Puromicin for 3 days before RNA extraction
Contributor(s) Neri F, Oliviero S
Citation(s) 24074865
Submission date Feb 25, 2013
Last update date Jan 16, 2019
Contact name Francesco Neri
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (8)
GSM1088306 ESC shGFP rep1
GSM1088307 ESC shGFP rep2
GSM1088308 ESC shDnmt3L1 rep1
This SubSeries is part of SuperSeries:
GSE44644 Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells
BioProject PRJNA190687

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44643_RAW.tar 15.8 Mb (http)(custom) TAR
GSE44643_non_normalized.txt.gz 2.4 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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