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Series GSE44642 Query DataSets for GSE44642
Status Public on Sep 27, 2013
Title Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells [MeDIP-seq]
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary The de novo DNA methyltransferase 3-like (Dnmt3L) is a catalytically inactive DNA methylase that has been previously shown to cooperate with Dnmt3a and Dnmt3b to methylate DNA. Dnmt3L is highly expressed in mouse embryonic stem cells (ESC) but its function in these cells is unknown. We here report that Dnmt3L is required for the differentiation of ESC into primordial germ cells (PGC) through activation of the homeotic gene Rhox5. By genome-wide analysis we found that Dnmt3L is a positive regulator of methylation at gene bodies of housekeeping genes and a negative regulator of methylation at promoters of bivalent genes. We demonstrate that Dnmt3L interacts with the Polycomb PRC2 complex in competition with the DNA methyl transferases Dnmt3a and Dnmt3b to maintain low the methylation level at H3H27me3 regions. Thus in ESC, Dnmt3L counteracts the activity of de novo DNA methylases to keep low the level of DNA methylation at developmental gene promoters.
Overall design Examination of 5mC in shGFP and shDnmt3L ESC by MeDIP-Seq
Contributor(s) Neri F, Oliviero S
Citation(s) 24074865
Submission date Feb 25, 2013
Last update date May 15, 2019
Contact name Francesco Neri
Organization name HuGeF
Street address Via Nizza 52
City Torino
State/province Italy
ZIP/Postal code 10126
Country Italy
Platforms (1)
GPL16173 Illumina HiScanSQ (Mus musculus)
Samples (2)
GSM1088304 MeDIP_shGFP
GSM1088305 MeDIP_shDnmt3L
This SubSeries is part of SuperSeries:
GSE44644 Dnmt3L-dependent regulation of DNA methylation promotes stem cells differentiation toward primitive germinal cells
BioProject PRJNA190686
SRA SRP018845

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Supplementary file Size Download File type/resource
GSE44642_RAW.tar 1.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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