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Series GSE44395 Query DataSets for GSE44395
Status Public on Jul 17, 2014
Title Effect of swim-training on caudal fin development in zebrafish larvae
Organism Danio rerio
Experiment type Expression profiling by array
Summary Genome-wide microarray analysis of the effects of swim-training on caudal fin development in zebrafish larvae. Zebrafish were subjected to swim-training from 5 days post fertilization (dpf) until 10 dpf. Subsequently, we performed a genome-wide microarray analysis on the caudal fins of control and trained fish at 10 dpf. The goal of the project was to investigate the effects of swim-training on the gene expression level during caudal fin development in zebrafish larvae.
 
Overall design Two-condition experiment: control vs trained fish. RNA was isolated from pooled caudal fins of 15 control fish (in duplo: pooled control samples (C2 and C3)) and of 15 trained fish (in duplo: pooled trained samples( T2 and T3)). Subsequently, each pooled RNA sample of control and trained caudal fins was labeled with Cy3 and Cy5 in order to correct for dye bias. We included a technical replicate of the labeled C2 and T2 samples.
 
Contributor(s) Fiaz AW
Citation A. W. Fiaz, K. M. Léon-Kloosterziel, J. L. van Leeuwen and S. Kranenbarg. Exploring the molecular link between swim-training and caudal fin development in zebrafish (Danio rerio) larvae. Journal of Applied Ichthyology. Volume 30, Issue 4, pages 753-761, August 2014. http://dx.doi.org/10.1111/jai.12510
Submission date Feb 19, 2013
Last update date Dec 17, 2015
Contact name ansa fiaz
E-mail(s) ansa.wasim@gmail.com
Organization name Wageningen University
Street address De Elst 1
City Wageningen
ZIP/Postal code 6708 WD
Country Netherlands
 
Platforms (1)
GPL14664 Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version)
Samples (6)
GSM1084301 Zebrafish_Control2_Cy3-Trained2_Cy5
GSM1084302 Zebrafish_Control2_Cy5-Trained2_Cy3
GSM1084303 Zebrafish_Control3_Cy3-Trained3_Cy5
Relations
BioProject PRJNA189745

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44395_RAW.tar 25.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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