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Series GSE44263 Query DataSets for GSE44263
Status Public on Mar 06, 2014
Title Transcriptomic effects of endocrine disruptors in zebrafish embryos
Organism Danio rerio
Experiment type Expression profiling by array
Summary The identification of endocrine disruptive properties of chemicals certain to enter the aquatic environment relies on toxicity tests with fish, assessing adverse effects on reproduction and sexual development. The demand for quick, reliable endocrine disruption (ED) assays favored the use of fish embryos as alternative test organisms. We investigated the application of a transcriptomics-based assay for estrogenic and anti-androgenic chemicals with zebrafish embryos. Two reference compounds, 17a-ethinylestradiol and flutamide, were tested to evaluate the effects on development and the transcriptome after 48h-exposures. Comparison of the transcriptome response with other estrogenic and anti-androgenic compounds (genistein, bisphenol A, methylparaben, linuron, prochloraz, propanil) showed commonalities and differences in regulated pathways, enabling us to classify the estrogenic and anti-androgenic potencies. This demonstrates that different mechanism of ED can be assessed already in fish embryos.
 
Overall design Newly fertilized embryos (<2hpf) were exposed for 48 hours to 0.65mg/l (EC10) and 0.8mg/l (EC20) 17-alpha ethinylestradiol (EE2), 1.2mg/l (EC10) and 1.4mg/l (EC20) flutamide, 8 mg/l (EC10)and 8.5mg/l (EC20) bisphenol A(BPA), 0.8 mg/l (EC10) and 1.1mg/l (EC20) propanil, 19.8mg/l (EC10) and 24.4mg/l (EC20) methylparaben, 1.2 mg/l (EC10) and 1.3mg/l (EC20) linuron as well as to 1.7mg/l (EC10) and 2 mg/l (EC20) prochloraz. Water controls were included in all studies and acetone solvent controls were applied when indicated (AC). For each study, four replicates (a,b,c,d) were performed per condition, each consisting of 24 pooled embryos.
 
Contributor(s) Schiller V, Wichmann A, Kriehuber R, Schäfers C, Fenske M
Citation(s) 24051129
Submission date Feb 12, 2013
Last update date Mar 06, 2014
Contact name Viktoria Schiller
E-mail(s) schiller@molbiotech.rwth-aachen.de
Phone 0049 (0)241 6085 12272
Organization name Fraunhofer IME
Department UNIFISH
Street address Forckenbeckstrasse 6
City Aachen
State/province NRW
ZIP/Postal code 52074
Country Germany
 
Platforms (1)
GPL7301 Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Probe Name version)
Samples (92)
GSM1081416 EE2 Solvent control A
GSM1081417 EE2 Solvent control B
GSM1081418 EE2 Solvent control C
Relations
BioProject PRJNA189265

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44263_RAW.tar 673.2 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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