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| Status |
Public on May 03, 2013 |
| Title |
Whole genome mRNA analysis of virus-specific CD8+ T cells expressing different levels of Id2-GFP following influenza virus infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We speculated that distinct levels of Id2 was deterministic in the transcriptional program of antigen-specific CD8+ T cells. To test this hypothesis, we subjected DbNP366-specific effector CD8+ T cells purified according to their differential expression of Id2-GFP (Id2-GFPint and Id2-GFPhigh) to microarray analysis and compared their gene expression profiles to the differentially expressed genes identified by comparing Id2-deficient and wild-type DbNP366-specific CD8+ T cells. This analysis revealed that the transcriptional program of CD8+ T cell differentiation is exquisitely sensitive to the concentration of Id2.
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| Overall design |
PR8-primed Id2gfp/gfp mice were challenged after one month with influenza intranasally HKx31 virus and analysed on day 9 for the expression of Id2-GFP in DbNP366+CD44+ CD8+ T cells. DbNP366-specific CD8+ T cells were then separated into virus-specific CD8+ T cells that expressed intermediate or high levels of GFP. Purified Id2-GFPintermediate (int) and Id2-GFPhigh DbNP366-specific CD8+ T cells were analysed by mRNA whole genome microarray. Three replicates of each group (Id2-GFPint or Id2-GFPhigh) were analysed.
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| Contributor(s) |
Masson F, Belz GT |
| Citation(s) |
23536629, 24723679 |
| Submission date |
Feb 07, 2013 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Moshe Olshansky |
| E-mail(s) |
olshansky@wehi.edu.au
|
| Organization name |
Walter & Eliza Hall Institute of Medical Research
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| Department |
Bioinformatics
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| Lab |
Speed
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA188769 |
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