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Series GSE44125 Query DataSets for GSE44125
Status Public on Jul 08, 2013
Title Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene
Organism Bacillus subtilis subsp. subtilis str. 168
Experiment type Expression profiling by array
Summary Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores.
 
Overall design A six chip study using total RNA extracted from three separate wild-type cultures of sporulating Bacillus subtilis 168 and three separate cultures of sporulating mutant strain, Bacillus subtilis 168 delta-prpE, in which prpE (yjbP BSU11630) gene coding for a protein phosphatase is deleted entirely. Each chip consists of four fields able to measure the expression level of 4,104 genes from Bacillus subtilis subsp. subtilis strain 168 NC_000964 with eight 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.
 
Contributor(s) Iwanicki A, Hinc K, Ronowicz A, Piotrowski A, Obuchowski M
Citation(s) 23824080
Submission date Feb 06, 2013
Last update date Jul 10, 2013
Contact name Adam Iwanicki
E-mail(s) maly@biotech.ug.gda.pl
Organization name Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk
Department Department of Medical Biotechnlogy
Lab Laboratory of Molecular Bacteriology
Street address Debinki 1
City Gdansk
ZIP/Postal code 80-211
Country Poland
 
Platforms (1)
GPL9817 TNO/NimbleGen 60-mer 071112 Bacillus subtilis subsp. subtilis strain 168 4x72K array
Samples (36)
GSM1079308 Bsub_prpE_60min_rep1
GSM1079309 Bsub_prpE_60min_rep2
GSM1079310 Bsub_prpE_60min_rep3
Relations
BioProject PRJNA188792

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE44125_RAW.tar 55.7 Mb (http)(custom) TAR (of CALLS, PAIR)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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