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| Status |
Public on Feb 01, 2013 |
| Title |
Evaluation of effect of PTEN gene deletion in mouse CD4+ Th1 clones after stimulation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
PTEN is thought to play a critical role in T cell activation by negatively regulating the PI3K signaling pathway important for cellular activation, growth, and proliferation. T cells from mice in which PTEN was conditionally deleted in the thymus were reported to display CD28-independent IL-2 production and relative resistance to anergy induction. However, such observations could have stemmed from alterations in T cell development due to early deletion in thymocytes. To directly eliminate PTEN in post-thymic T cells, we utilized CAR Tg x PTENflox/flox mice which enabled gene deletion using a Cre adenovirus in vitro. Gene expression profiling revealed a small subset of induced genes that were augmented upon PTEN deletion and T cell stimulation. Our results indicate that deletion of PTEN can augment the activation of post-thymic T cells. Nonetheless, PTEN inhibition may be a viable target for immune potentiation due to increased cytokine production by activated CD4+ cells.
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| Overall design |
It was of interest to determine the spectrum of transcripts regulated by PTEN using gene expression profiling. To this end, CAR Tg x PTENflox/flox Th1 clones were either treated with adeno-Cre or adeno-EV, then confirmed to have PTEN protein abscence by western blot, then stimulated for 6 hours with anti-CD3/anti-CD28 mAb- coated beads and analyzed (2 total conditions). The experiment was replicated so there are 2 samples for each condition.
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| Contributor(s) |
Locke FL, Gajewski TF, Zha Y |
| Citation(s) |
23851688 |
| |
| Submission date |
Jan 31, 2013 |
| Last update date |
Feb 11, 2019 |
| Contact name |
yuanyuan zha |
| E-mail(s) |
yzha@uchicago.edu
|
| Phone |
772-702-4812
|
| Fax |
773-702-7195
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| Organization name |
University of Chicago
|
| Department |
Department of Pathology
|
| Street address |
910 E. 58th St. MKL051
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (4)
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| GSM1074697 |
T cells_empty virus transduced_stimulated_6 hours_rep1 |
| GSM1074698 |
T cells_Cre virus transduced_stimulated_6 hours_rep1 |
| GSM1074699 |
T cells_empty virus transduced_stimulated_6 hours_rep2 |
| GSM1074700 |
T cells_Cre virus transduced_stimulated_6 hours_rep2 |
|
| Relations |
| BioProject |
PRJNA188179 |
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