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Series GSE43876 Query DataSets for GSE43876
Status Public on Jun 13, 2014
Title Mtg16 regulates E protein activity and lineage specification in dendritic cell development (ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary E protein transcription factors specify major immune cell lineages including lymphocytes and interferon-producing plasmacytoid dendritic cells (pDCs). Corepressors of the ETO family can bind to and block transactivation by E proteins, but the physiological role of these interactions remained unclear. We report that ETO protein Mtg16 binds chromatin primarily through the pDC-specific E protein E2-2 in human pDCs. Mtg16-deficient mice showed impaired pDC development and functionality, whereas the specification of the classical dendritic cells (cDCs) was enhanced. The deletion of Mtg16 caused aberrant expression of E protein antagonist Id2 in pDCs. Thus, Mtg16 acts as a cofactor of E2-2 to promote pDC differentiation and restrict cDC development, revealing an unexpected positive role of ETO proteins in E protein activity.
Overall design Analysis of E2-2 and Mtg16 immunoprecipitated chromatin from CAL-1 cell line.
Contributor(s) Ghosh HS, Reizis B
Citation(s) 24980046
Submission date Jan 29, 2013
Last update date May 15, 2019
Contact name Hiyaa Singhee Ghosh
Organization name Columbia University Medical Center
Street address 701 W 168th Street
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (3)
GSM1072709 CAL-1 input
GSM1072710 CAL-1-Mtg16-ChIP
GSM1072711 CAL-1-E2-2-ChIP
This SubSeries is part of SuperSeries:
GSE43963 Mtg16 regulates E protein activity and lineage specification in dendritic cell development
BioProject PRJNA188233
SRA SRP018349

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Supplementary file Size Download File type/resource
GSE43876_RAW.tar 720.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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