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Series GSE43743 Query DataSets for GSE43743
Status Public on Dec 13, 2013
Title ChIP-Seq of transcriptional regulators in MM1S cells upon small molecule treatment.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Discovery of the genome-wide location of proteins using ChIP-Seq has allowed global mapping of the key transcription factors and chromatin regulators that control gene expression programs in various cells. Many DNA-associated processes are targeted for disease therapy. This study investigates the functions of small molecule therapeutics that target DNA-associated processes involved of CDK9 and BRD4.
Overall design Genomic DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. ChIP was performed using an antibody against RNAP2, BRD2, BRD3, BRD4, CDK8, CDK9, H3K27me3, H3K20me3, and CTCF using whole cell extract (WCE) as a background control.
Contributor(s) Lars A, Hoke H, Peter R, Young RA
Citation(s) 24336317
Submission date Jan 24, 2013
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (21)
GSM1070124 MM1S_BRD4
GSM1070125 MM1S_CTCF
GSM1070127 MM1S_RNAPol2_DMSO_forCdk9i
BioProject PRJNA187203
SRA SRP018149

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43743_RAW.tar 22.9 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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