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Series GSE43350 Query DataSets for GSE43350
Status Public on Mar 03, 2013
Title Lineage-specific functions of Bcl6  in immunity and inflammation are mediated through distinct biochemical mechanisms
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcription factor Bcl6 orchestrates the germinal center reaction through its actions in B and T cells, and regulates inflammatory signaling in macrophages. We report that genetic replacement by mutant Bcl6, which cannot bind corepressors to its BTB domain, disrupted  germinal center formation and immunoglobulin affinity maturation, due to a defect in B cell  proliferation and survival. In contrast, BTB loss of function had no effect on T follicular helper cell differentiation and function, nor other T helper subsets. Bcl6 null mice displayed a lethal inflammatory phenotype, whereas BTB mutant mice experienced normal healthy lives with no inflammation. Bcl6 repression of inflammatory responses in macrophages was accordingly independent of the BTB domain repressor function. Bcl6 thus mediates its actions through lineage-specific biochemical functions.
Overall design ChIP-seq for Bcl6, SMRT and BCOR in germinal center B cells
Contributor(s) Hatzi K, Melnick A
Citation(s) 23455674
Submission date Jan 08, 2013
Last update date May 15, 2019
Contact name Katerina Hatzi
Organization name WCMC
Department Hematology/Oncology
Lab Ari Melnick
Street address 413 E 69th Street, BB-1462
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
BioProject PRJNA185984
SRA SRP018041

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Supplementary file Size Download File type/resource
GSE43350_RAW.tar 1.9 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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