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Series GSE43118 Query DataSets for GSE43118
Status Public on Dec 26, 2012
Title Protein Determinants of Meiotic DNA Break Hotspots (dataset 1)
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Meiotic recombination, crucial for proper chromosome segregation and genome evolution, is initiated by programmed DNA double-strand breaks (DSBs) in budding and fission yeasts and likely all sexually reproducing species. In fission yeast, DSBs occur up to several hundred times more frequently at special sites, called hotspots, than in other regions of the genome. What distinguishes hotspots from cold regions is a major unsolved problem, although transcription factors determine some hotspots. We report here the discovery that three coiled-coil proteins -- Rec25, Rec27, and Mug20 -- bind essentially all hotspots with unprecedented, high specificity even without DSB formation. These small proteins are components of linear elements, are related to synaptonemal complex proteins, and are essential for nearly all DSBs at most hotspots. Our results indicate that these hotspot determinants activate or stabilize the DSB-forming protein Rec12 (Spo11 homolog) rather than promote its binding to hotspots. We propose here a new paradigm for hotspot determination and crossover control by linear element proteins.
Overall design Meiotic DSB analysis by immunoprecipitation of Rec12 covalently linked to DNA (no exogenous crosslinking agent used) in WT and various mutants; one mutant repeated.
Contributor(s) Fowler KR, Smith GR
Citation(s) 23395004
Submission date Dec 21, 2012
Last update date Mar 27, 2013
Contact name Kyle Fowler
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences Division
Lab Smith Lab
Street address 1100 Fairview Ave. N.
City Seattle
State/province Washington
ZIP/Postal code 98109
Country USA
Platforms (1)
GPL16383 Agilent-015424 S. pombe Whole Genome ChIP-on-Chip Microarray 4x44K (Probe Name version - A_86_Pnnnn probes)
Samples (5)
GSM1056914 DSBs_WT_1
GSM1056915 DSBs_rec27d_1
GSM1056916 DSBs_rec8d_1
This SubSeries is part of SuperSeries:
GSE43122 Protein Determinants of Meiotic DNA Break Hotspots
BioProject PRJNA184595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43118_RAW.tar 21.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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