NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE43109 Query DataSets for GSE43109
Status Public on Oct 31, 2014
Title The role of HIF-1α and HIF-2α in hypoxic and M2-polarized macrophages
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Macrophages (MФ) skewn towards a regulatory-like phenotype are known to promote tumor progression. They tend to accumulate in hypoxic tumor areas and activate hypoxia-inducible factors (HIF-1 and HIF-2). HIFs are known to alter the gene expression profile of MФ. To define direct HIF-1 and HIF-2 target genes in hypoxic and IL-10 treated human MФ we used chromatin immuno-precipitation-sequencing (ChIP-seq) and microarray analysis on a genome-wide scale.
 
Overall design ChIP-seq of HIF1a and HIF2a in human macrophages under hypoxic and normoxic conditions with and without Interleukin-10 treatment
 
Contributor(s) Tausendschön M, Dehne N, Brüne B, Schmidl C, Rehli M
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 21, 2012
Last update date May 15, 2019
Contact name Michael Rehli
E-mail(s) michael.rehli@klinik.uni-r.de
Organization name University Hospital Regensburg
Department Internal Med III
Street address F.-J.-Strauss-Allee 11
City Regensburg
ZIP/Postal code 93042
Country Germany
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (16)
GSM1056829 HIF1a_Normoxy 1
GSM1056830 HIF1a_Normoxy 2
GSM1056831 HIF1a_Hypoxy 1
Relations
SRA SRP017659
BioProject PRJNA185268

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE43109_RAW.tar 2.7 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap