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Series GSE42819 Query DataSets for GSE42819
Status Public on Mar 29, 2013
Title The landscape of RNA polymerase II transcription initiation in C. elegans reveals a novel regulatory architecture
Organism Caenorhabditis elegans
Experiment type Expression profiling by high throughput sequencing
Summary It has recently been shown that RNA Polymerase II transcription is far more extensive than previously thought, much of it not associated with protein-coding genes. To investigate this phenomenon, we determined the genome-wide landscape of RNA Polymerase II transcription initiation and elongation in C. elegans. We identify 73,500 distinct clusters of transcription initiation and find that initiation is often bidirectional. Strikingly, the majority of initiation events occur in regions with enhancer-like chromatin signatures. We also assign transcription initiation sites to 7691 protein coding genes, the majority previously unknown because of trans-splicing.
Overall design Through mapping RNA PolII initiation (short capped RNAs) and elongation (long capped RNAs), we provide identification of transcription start sites.
Contributor(s) Ahringer J
Citation(s) 23550086
Submission date Dec 10, 2012
Last update date May 15, 2019
Contact name Thomas A Down
Organization name University of Cambridge
Department WT/CRUK Gurdon Institute
Street address Tennis Court Road
City Cambridge
ZIP/Postal code CB2 1QN
Country United Kingdom
Platforms (2)
GPL13657 Illumina HiSeq 2000 (Caenorhabditis elegans)
GPL13776 Illumina Genome Analyzer IIx (Caenorhabditis elegans)
Samples (5)
GSM1050558 short_capRNA_1
GSM1050559 short_capRNA__1_tech
GSM1050560 short_capRNA_2
BioProject PRJNA183503
SRA SRP017486

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Supplementary file Size Download File type/resource
GSE42819_RAW.tar 98.3 Mb (http)(custom) TAR (of BB, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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