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| Status |
Public on Dec 11, 2012 |
| Title |
Microarray gene expression profiling of aortic genes of APOE-deficient mice receiving atherosclerosis treatment with the antioxidant vitamin E |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hypercholesterolemic APOE-deficient mice are a widely used experimental model of atherosclerosis and increased generation of reactive oxygen species (ROS) is a prominent feature of atherosclerosis development. To study the impact of ROS on atherogenesis, we treated APOE-deficient mice for 7 months with the antioxidant vitamin E (2000 IU/kg diet) and performed whole genome microarray gene expression profiling of aortic genes. Microarray gene expression profiling was performed of whole aortas isolated from vitamin E-treated APOE-deficient relative to untreated APOE-deficient mice with overt atherosclerosis, and nontransgenic B6 control mice. Microarray gene expression profiling revealed that vitamin E treatment prevented atherosclerosis-related gene expression changes of the aortic intima and media.
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| Overall design |
Microarray gene expression profiling was performed of whole aortas isolated from APOE-deficient mice with atherosclerosis relative to vitamin E-treated APOE-deficient mice, and nontransgenic B6 control mice. Three study groups were analyzed, i.e. 8 months-old untreated APOE-deficient mice with overt atherosclerosis, age-matched APOE-deficient mice treated for 7 months with the antioxidant vitamin E (2000 IU/kd diet), and nontransgenic B6 control (C57BL/6J) mice. Two biological replicates were made of each group, and total RNA of three aortas was pooled for one gene chip. The study complements microarray study GSE19286.
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| Contributor(s) |
Abd Alla J, Quitterer U |
| Citation(s) |
23801967 |
| |
| Submission date |
Dec 10, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Ursula Quitterer |
| E-mail(s) |
ursula.quitterer@pharma.ethz.ch
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| Phone |
+41-446329801
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| Organization name |
ETH Zurich
|
| Department |
Molecular Pharmacology
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
CH-8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM478606 |
Aorta_APOE-deficient with atherosclerosis_1 |
| GSM478607 |
Aorta_APOE-deficient with atherosclerosis_2 |
| GSM478610 |
Aorta_non-transgenic C57BL/6J control_1 |
| GSM478611 |
Aorta_non-transgenic C57BL/6J control_2 |
| GSM1050380 |
Aorta-APOE-deficient and vitamin E treatment-rep1 |
| GSM1050381 |
Aorta-APOE-deficient and vitamin E treatment-rep2 |
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| Relations |
| BioProject |
PRJNA183457 |
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