|
| Status |
Public on Dec 05, 2012 |
| Title |
Genome wide maps of p53 binding in IMR90 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Oncogene-induced senescence is an anti-proliferative stress response program that acts as a fail-safe mechanism to limit oncogenic transformation and is regulated by the retinoblastoma protein (RB) and p53 tumor suppressor pathways. We identify the atypical E2F family member E2F7 as the only E2F transcription factor potently upregulated during oncogene-induced senescence, a setting where it acts in response to p53 as a direct transcriptional target. Once induced, E2F7 binds and represses a series of E2F target genes and cooperates with RB to efficiently promote cell cycle arrest and limit oncogenic transformation. Disruption of RB triggers a further increase in E2F7, which induces a second cell cycle checkpoint that prevents unconstrained cell division despite aberrant DNA replication. Mechanistically, E2F7 compensates for the loss of RB in repressing mitotic E2F target genes.
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| |
|
| Overall design |
Examination of p53 binding in either growing or senescent IMR90 cells with different hairpins.
|
| |
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| Contributor(s) |
Aksoy O, Wang X, Chicas A, Lowe SW, Zeng T |
| Citation(s) |
22802529 |
| |
| Submission date |
Dec 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Ozlem Aksoy |
| E-mail(s) |
merto@mskcc.org
|
| Phone |
917-930-1718
|
| Organization name |
CSHL
|
| Department |
Watson School of Biological Sciences
|
| Lab |
Scott Lowe's lab
|
| Street address |
415 East 68th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA182950 |
| SRA |
SRP017415 |
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