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Series GSE42666 Query DataSets for GSE42666
Status Public on Aug 01, 2013
Title Expression data from Tet2-hypomorph (knockdown) and/or Ezh2-null Lineage-Sca-1+c-Kit+ (LSK) cells and granulocyte-macrophage progenitors (GMPs)
Organism Mus musculus
Experiment type Expression profiling by array
Summary PcG proteins form the polycomb repressive complexes (PRC) 1 and 2, functioning as transcriptional repressors through histone modifications. They have been implicated in the maintenance of self-renewing somatic and cancer stem cells. PcG genes have been characterized as tumor suppressor genes as exemplified by somatic inactivating mutations of EZH2, a gene encoding histone methyltransferase in PRC2, in myeloid malignancy. Mice deficient for Tet2 have been reported to recapitulate some aspects of myeloid malignancies. We evaluated the Ezh2-deficient mice and also tested the impact of concurrent depletion of Ezh2 and Tet2 on hematopoiesis.
 
Overall design Purified LSK cells and GMPs from BM of recipient mice repopulated with wild-type, Tet2KD/KD, Ezh2-/- and Tet2KD/KDEzh2-/- fetal liver cells were subjected to RNA extraction and hybridization on Agilent microarrays.
 
Contributor(s) Iwama A, Muto T
Citation(s) 24218139
Submission date Dec 02, 2012
Last update date Jul 19, 2017
Contact name ATSUSHI IWAMA
E-mail(s) aiwama@faculty.chiba-u.jp
Phone 81-43-226-2187
Fax 81-43-226-2191
Organization name Graduate School of Medicine, Chiba University
Department Department of Cellular and Molecular Medicine
Street address 1-8-1 Inohana, Chuo-ku
City Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (12)
GSM1047667 1-LSK_WT
GSM1047668 2-LSK_Tet2_SKO
GSM1047669 3-LSK_Ezh2_SKO
Relations
BioProject PRJNA182595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42666_RAW.tar 45.5 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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