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Series GSE42619 Query DataSets for GSE42619
Status Public on Jan 01, 2013
Title Gene expression profiles of MCF7 with with the following NR ligands: estradiol/E2 (ERĪ± agonist), AM580 (RARA agonist), CD437 (RARG agonist), ATRA (RARA/RARG agonist), GW0742 (PPARD agonist), R5020 (PGR agonist), and dexamethasone (GR/NR3C1 agonist).
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Altered gene expression patterns in human diseases reflect perturbations in the transcriptional networks that regulate cellular state. In breast cancer, Nuclear Receptors (NRs) play a prominent role in governing gene expression. NRs have prognostic utility and are therapeutically important targets. In order to understand the function of nuclear receptors in breast cancer, we treated MCF7 cells with 6 nuclear receptor agonist, and performed expression arrrays with three biological replicates each.
 
Overall design MCF-7 cells were cultured for 48 hours in medium with 10% charcoal-stripped FBS, and then were treated with 6 diffferent NR ligand (E2, AM580, CD437, ATRA, R5020, DEX) separately for 24h before collection and processing for microarrays. Three biological replicates were collected and processed for both control and treatment. with the 100nM GW0742 (PPARD specific ligand) and 100nM ATRA (RARs ligand), then collected after 24h for microarray processing. Controls were non-treated MCF7 cells. Both treatments and controls have three biological replicates.
 
Contributor(s) Kittler R, Zhou J, Hua S, Ma L, Liu Y, Cheng C, Gerstein M, White K
Citation(s) 23375374
Submission date Nov 29, 2012
Last update date Feb 22, 2018
Contact name Jie Zhou
E-mail(s) jiezhou@uchicago.edu
Phone 3123163491
Organization name University of Chicago IGSB
Street address 900 East 57th Street, KCBD 10100
City Chicago
State/province ILLINOIS
ZIP/Postal code 60637
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (33)
GSM1045863 E2_Control_rep1
GSM1045864 E2_Control_rep2
GSM1045865 E2_Control_rep3
Relations
BioProject PRJNA182369

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42619_RAW.tar 285.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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