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Series GSE42459 Query DataSets for GSE42459
Status Public on Aug 01, 2013
Title CD8+ T-cell memory bears the transcriptional imprint of progressive differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The generation of CD8+ T-cell memory is an important aim of immunization. While several distinct subsets of CD8+ T-cell memory have been described, the lineage relationships between effector (EFF), effector memory (EM) and central memory (CM) T cells remain contentious. Specifically, there is contradictory experimental evidence to support both the linear (Naive>EFF>EM>CM) and progressive differentiation (Naive>CM>EM>EFF) models. In this study, we applied a systems biology approach to examine global transcriptional relationships between the three major CD8+ T cell subsets arising endogenously as a result of vaccination with three different prime-boost vaccine regimens. Differential gene expression analysis and principle component analysis revealed that central memory cells were more closely related to naive T cells than both effector memory and effector cells. When the transcriptional relationships between subsets were enriched in an unbiased fashion with known global transcriptional changes that result when T-cells repeatedly encounter antigen, our analysis favored a model whereby cumulative antigenic stimulation drives differentiation specifically from Naive > CM > EM > EFF. These findings provide an insight into the lineage relationship between mature CD8+ T-cell subsets and will help in the rational design of vaccines aimed at generating effective immune responses against infections and cancer.
Overall design Effector (EFF), effector memory (EM), central memory (CM) and naive CD8+ T cells from mice spleen. Memory subset arise endogenously as a result of vaccination with three different prime-boost vaccine regimens: DNA-rAd5, rAd5-rAd5 and rAd5-rLCMV.
Contributor(s) Roychoudhuri R, Lefebvre F, Honda M, Klebanoff CA, Sukumar M, Pan L, Goulet J, Kettaf N, Haddad EK, Gattinoni L, Nabel GJ, Restifo NP, Sékaly RP, Flatz L, Wilkinson PA
Citation(s) 21422297, 25446821
Submission date Nov 21, 2012
Last update date Feb 07, 2017
Contact name Peter A Wilkinson
Organization name Case Western Reserve University
Department Pathology / Systems Biology & Bioinformatics
Street address 2103 Cornell Road
City Cleveland
State/province Ohio
ZIP/Postal code 44120
Country USA
Platforms (1)
GPL8389 Illumina Mouse-8 Expression BeadChip
Samples (67)
GSM1040504 EFF DNA_rAd 54
GSM1040505 EFF DNA_rAd 55
GSM1040506 EFF DNA_rAd 56
BioProject PRJNA182004

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Supplementary file Size Download File type/resource
GSE42459_non-normalized.txt.gz 6.2 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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