|
| Status |
Public on Oct 29, 2012 |
| Title |
Prolactin-induced protein (PIP) regulates proliferation of luminal A type breast cancer cells in a hormone-independent manner |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
RNA was extracted from cells using Aurum Total RNA kit from Bio-Rad Laboratories, Inc., Hercules, CA following the manufacturer’s recommendations. Gene expression profiling was performed using the BeadChip HumanHT-12 v4 Expression kit from Illumina®, which contains 47,231 gene-probes (Illumina® Inc., San Diego, CA). The raw signal intensities were imported and analyzed using the GenomeStudio® data software. After background subtraction and normalization, the signal intensity values were exported to the Partek® genomics expression analysis suite using “Partek's Report Plug-in” option in the GenomeStudio® software. Differentially expressed genes in the dox- versus vehicle-treated samples were identified using the “gene expression” workflow in the Partek® software.
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| |
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| Overall design |
T47D cells hoaboring a doxycycline inducible shPIP construct were treated for 24 or 48 hours with 250 ng/mL of doxycycline and their mRNAs analyzed in biological triplicates (a total of 12 samples) using Illumina‘s HumanHT-12 v4 BeadChips. The samples for each time point comprised of three samples each for doxycycline or equal volume of water as vehicle control.
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| |
|
| Contributor(s) |
Baniwal SK |
| Citation(s) |
23755096 |
| Submission date |
Oct 28, 2012 |
| Last update date |
Sep 16, 2019 |
| Contact name |
sanjeev k baniwal |
| E-mail(s) |
sbaniwal@gmail.com
|
| Organization name |
University of Southern California
|
| Department |
Orthopaedics
|
| Street address |
2220 Alcazar street
|
| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (12)
|
| GSM1027084 |
48 Hour vehicle treated_6116750018_D |
| GSM1027085 |
24 Hour vehicle treated_6116750018_I |
| GSM1027086 |
24 Hour vehicle treated_6116750018_J |
| GSM1027087 |
24 Hour vehicle treated_6116750018_K |
| GSM1027088 |
24 Hour Doxycycline treated_6116750018_L |
| GSM1027089 |
24 Hour Doxycycline treated_6116750017_I |
| GSM1027090 |
24 Hour Doxycycline treated_6116750017_J |
| GSM1027091 |
48 Hour Doxycycline treated_6116750017_K |
| GSM1027092 |
48 Hour Doxycycline treated_6116750017_L |
|
| Relations |
| BioProject |
PRJNA178414 |
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