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Series GSE41860 Query DataSets for GSE41860
Status Public on Dec 31, 2013
Title Time-course expression data during assurance of the chronological longevity by caloric restriction in budding yeast
Platform organisms Schizosaccharomyces pombe; Saccharomyces cerevisiae
Sample organism Saccharomyces cerevisiae BY4741
Experiment type Expression profiling by array
Summary Caloric restriction (CR) is the only non-genetic intervention to retard aging and increase longevity in a variety of species. It is important to understand the fundamental mechanism by which CR extends lifespan that remains elusive. Owing to well-established genomic tools and convenience of culture system, we used a single cell organism, Saccharomyces cerevisiae, to clarify the mechanisms of CR.
In order to identify genes responsible for CR-mediated longevity, we performed microarray experiments across the longevity assurance time-points.
Overall design Since the CR-treated cells obtained the longevity potential around 12 hours after inoculation and the strength of potential gradually increased up to 48 hours, we concluded that the changes across these time-points must be a critical need for assurance of longevity by CR. For preparation of total RNA, yeast cells under control (2% glucose) and CR (0.5% glucose) conditions were harvested at 12h, 18h, 24h and 48h after inoculation. After total RNA extraction, we performed Affymetrix Yeast GeneChip 2.0 array for biological triplicate repeats according to the manufacturer's instructions.
Contributor(s) Lee C, Choi K
Citation(s) 24126084
Submission date Oct 26, 2012
Last update date Feb 21, 2017
Contact name Cheol-Koo Lee
Phone 82-2-3290-3008
Organization name Korea University
Department Biotechnology
Lab Functional Genomics
Street address Korea University, Biotechnology #318 West building, Anam-dong, Seongbuk-gu
City Seoul
ZIP/Postal code 136-701
Country South Korea
Platforms (1)
GPL2529 [Yeast_2] Affymetrix Yeast Genome 2.0 Array
Samples (24)
GSM1026409 Yeast_12h in 2% glucose_rep1
GSM1026410 Yeast_12h in 2% glucose_rep2
GSM1026411 Yeast_12h in 2% glucose_rep3
BioProject PRJNA178324

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Supplementary file Size Download File type/resource
GSE41860_RAW.tar 20.4 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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