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| Status |
Public on Oct 31, 2012 |
| Title |
Expression data from HeLa cells treated with Casiopeina Cas-II-gly |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application.
Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly.
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| Overall design |
The cervix-uterine cell line HeLa was maintained at 37C in 5% CO2 under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h. HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.
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| Contributor(s) |
Valencia-Cruz AI, Uribe-Figueroa LI, Galindo-Murillo R, Baca-Lopez K, Gutierrez AG, Vazquez-Aguirre A, Ruiz-Azuara L, Hernandez-Lemus E, Mejia C |
| Citation(s) |
23382936 |
| Submission date |
Oct 24, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Karol Baca Lopez |
| E-mail(s) |
kbacal@uaemex.mx
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| Organization name |
National Institute of Genomic Medicine
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| Street address |
Periferico sur
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| City |
Mexico City |
| ZIP/Postal code |
14610 |
| Country |
Mexico |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| GSM1025052 |
HeLa treated with Cas-II-gly, biological rep1 |
| GSM1025053 |
HeLa treated with Cas-II-gly, biological rep2 |
| GSM1025054 |
HeLa treated with Cas-II-gly, biological rep3 |
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| Relations |
| BioProject |
PRJNA178551 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41827_RAW.tar |
27.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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