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Series GSE41827 Query DataSets for GSE41827
Status Public on Oct 31, 2012
Title Expression data from HeLa cells treated with Casiopeina Cas-II-gly
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Copper-based chemotherapeutic compounds Casiopeinas, have been presented as able to promote selective programmed cell death in cancer cells, thus being proper candidates for targeted cancer therapy. DNA fragmentation and apoptosis -in a process mediated by reactive oxygen species- for a number of tumor cells, have been argued to be the main mechanisms. However, a detailed functional mechanism (a model) is still to be defined and interrogated for a wide variety of cellular conditions; before establishing settings and parameters needed for their wide clinical application.
Microarrays were used to determine the expression profile from HeLa cells in order to propose a model for the role played by intrinsic apoptosis triggered by the oxidative stress caused by Cas-II-gly.
Overall design The cervix-uterine cell line HeLa  was maintained at 37C in 5% CO2  under sterile conditions in Dulbecco's modified Eagle medium (DMEM, Sigma), supplemented with 10% fetal bovine serum (Sigma). Cells were treated with Cas II-gly in 96-well microplates by 6 h.  HeLa cells whole genome gene expression experiments (triplicates for cases/controls) were performed in total mRNA extracted under the GPL570 protocol.
Contributor(s) Valencia-Cruz AI, Uribe-Figueroa LI, Galindo-Murillo R, Baca-Lopez K, Gutierrez AG, Vazquez-Aguirre A, Ruiz-Azuara L, Hernandez-Lemus E, Mejia C
Citation(s) 23382936
Submission date Oct 24, 2012
Last update date Mar 25, 2019
Contact name Karol Baca Lopez
Organization name National Institute of Genomic Medicine
Street address Periferico sur
City Mexico City
ZIP/Postal code 14610
Country Mexico
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM1025049 HeLa control, biological rep1
GSM1025050 HeLa control, biological rep2
GSM1025051 HeLa control, biological rep3
BioProject PRJNA178551

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Supplementary file Size Download File type/resource
GSE41827_RAW.tar 27.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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