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Series GSE4182 Query DataSets for GSE4182
Status Public on Nov 02, 2006
Title Genetic background of the polygenic neural tube defect
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In polygenic disorders we do not know exactly, how many genes are involved in the pathomechanism, but the analysis of fetal gene expression can get us closer to the solution. In our study we were searching for the genetic background of the polygenic neural tube defect, which is the second most common birth defect in the world (1 in 1000 live births). Our data revealed novel candidate genes, like SLAP, LST1 and BENE, which can play an important role in the pathogenesis of neural tube defects. We created a data warehouse from the results, suitable for further analysis. This study also demonstrates that a routinely collected amount of amniotic fluid (as small as 6 mL) is enough to successfully hybridize isolated RNA to expression arrays, making the ability to use the technique from normally collected amniotic fluid samples.
Keywords: Prenatal gene expression signature
 
Overall design We report an in vivo study in living human fetuses using oligonucleotide microarray analysis of fetal mRNA isolated from amniocytes, which were collected by amniocentesis. RNA was successfully isolated, amplified, labeled, and hybridized to whole-genome Affymetrix transcript arrays.
 
Citation(s) 17008366
Submission date Feb 06, 2006
Last update date Mar 25, 2019
Contact name Balazs Györffy
E-mail(s) zsalab2@yahoo.com
Organization name Hungarian Academy of Sciences
Street address Bókay 53
City Budapest
ZIP/Postal code H1083
Country Hungary
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (9)
GSM94597 MV1
GSM94598 MV11
GSM94599 MV12
Relations
BioProject PRJNA95143

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4182_RAW.tar 73.6 Mb (http)(custom) TAR (of CEL)

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