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| Status |
Public on Jun 26, 2013 |
| Title |
Differential expression in Wn5a and vector transduced 4T1 cells. [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A highly metastatic breast cancer cell line, 4T1, was used to generate stable Wnt5a expressing and vector only control cells. Cells were generated using lentivirus infection and selection with blasticidin. Expression of Wnt5a was confirmed using western blot. Cell behaviour was characterized. Wnt5a expressing cells exhibited reduced migration in a transwell assay and reduced metastasis in a tail vein injection assay. Growth was not significantly affected.
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| Overall design |
One sample of each condition.
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| |
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| Contributor(s) |
Serra R, Crossman D |
| Citation(s) |
23484019 |
| |
| Submission date |
Oct 23, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Rosa Serra |
| E-mail(s) |
rserra@uab.edu
|
| Phone |
205-934-0842
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Cell Biology
|
| Lab |
Serra
|
| Street address |
1918 University Blvd. 660 MCLM
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294-0005 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE41793 |
Differential expression in Wn5a and vector transduced 4T1 cells. |
|
| Relations |
| BioProject |
PRJNA178237 |
| SRA |
SRP016627 |
