|
| Status |
Public on Oct 20, 2012 |
| Title |
Response of Rpp4-silenced plants to soybean rust |
| Organism |
Glycine max |
| Experiment type |
Expression profiling by array
|
| Summary |
Experimental design: 1 genotypes: PI- (resistant USDA Plant Introduction (PI459025B) line containing SBR Rpp4 resistance gene) 2 treatments: Rub inoculation with BPMV-VIGS construct targeting the LRR region of Rpp4 candidate gene. This BPMV construct and the plant tissue used for microarray was described in Meyer et al. (2009) Plant Physiol. 150:295-307. Control plants were inoculated with empty BPMV vector. At three weeks after BPMV inoculation, all plants were inoculated with Soybean rust (Phakopsora pachyrhizi) isolate LA04-1. LA04-1 is a single spore isolate maintained at the Foreign Disease Weed Science Research Unit. 3 replications 1 time point: 14 days after inoculation TOTAL: 5 Affymetrix GeneChip(R) Soybean Genome Arrays LA04-1 treatment: 500,000 spores per ml in 0.01% Tween 20 ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Michelle Graham. The equivalent experiment is GM51 at PLEXdb.]
|
| |
|
| Overall design |
treatment: BPMV-LRR(2-replications); treatment: BPMV empty vector(3-replications)
|
| Web link |
http://www.plexdb.org/modules/PD_browse/experiment_browser.php?experiment=GM51
|
| |
|
| Contributor(s) |
Graham M, Graham M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 19, 2012 |
| Last update date |
Nov 22, 2016 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4592 |
[Soybean] Affymetrix Soybean Genome Array |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA178023 |
Less...
More...