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Series GSE41707 Query DataSets for GSE41707
Status Public on Nov 07, 2012
Title Expression profiles in testis of mouse inter-subspecific hybrids
Organism Mus musculus
Experiment type Expression profiling by array
Summary According to Dobzhansky-Muller model, hybrid sterility is a consequence of independent evolution of related taxa resulting in incompatible interaction during gametogenesis of their hybrids. We proposed that asynapsis of heterospecific chromosomes in meiotic prophase provides a general and recurrently evolving trigger for the meiotic arrest of interspecific F1 hybrids. We used genome-wide expression profiling to quantify misexpression of Chr X and Chr Y genes.
 
Overall design The total RNA (20–30 ng) samples were extracted from 14.5 days juvenile testes (pool of 4 samples), converted to cDNA using the Affymetrix 3 IVT Express Kit and hybridized to Affymetrix Mouse Gene 1.0ST GeneChips (core facility of the Institute of Molecular Genetics AS CR, Prague, Czech Rep.) We visually checked intensity histograms, boxplots and PCA graph and excluded one PWDxPWK sample that was a clear outlyer on all QC graphs (not shown).
Web link http://lion.img.cas.cz/user/simecek/chips/Ovmxw643tHA28AUZ/
 
Contributor(s) Simecek P, Bhattacharyya T, Forejt J
Citation(s) 23329330
Submission date Oct 19, 2012
Last update date Mar 04, 2019
Contact name Petr Simecek
E-mail(s) simecekp@img.cas.cz
Phone +420 24106 2155
Organization name Institute of Molecular Genetics of the ASCR, v. v. i.
Department Department of Mouse Molecular Genetics
Lab Jiri Forejt's lab
Street address Vídeňská 1083
City Prague
State/province Not Applicable
ZIP/Postal code 14220
Country Czech Republic
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (23)
GSM1022966 PWD juvenile testes 1
GSM1022967 PWD juvenile testes 2
GSM1022968 (B6xBALB/C)F1 juvenile testes 1
Relations
BioProject PRJNA178002

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41707_RAW.tar 91.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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