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Series GSE41684 Query DataSets for GSE41684
Status Public on Oct 11, 2015
Title Precision Cut Lung Slices: A Novel Method for Examining Respiratory Diseases
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bacterial infections and cigarette smoking have been linked to exacerbations of respiratory disease including severe asthma and chronic obstructive pulmonary disease (COPD). Epidemiological studies have also shown increased incidences of respiratory tract infections even in young smokers. We have previously shown that cigarette smoke exacerbates the inflammatory response to influenza A virus (PLoS ONE. 2010 Oct 12;5(10): e13251) and nontypeable Haemophilus influenzae (Am J Respir Crit Care Med. 2009 Apr 15;179(8):666) infection. While it is well understood that cigarette smoke impairs respiratory host defense, little is known with respect to mechanisms driving the outcome of infection in smoke-exposed mice. In this study, we attempt to characterize the antibacterial responses of lung resident cells from smoke-exposed mice to TLR agonists and live pathogens by gene expression profiling and to compare similarities and differences of inflammatory profiles between mouse and man.
 
Overall design C57BL/6 mice were exposed to room air or cigarette smoke for four days. Mice were exposed to twelve cigarettes (2R4 reference cigarettes; Tobacco and Health Research Institute, University of Kentucky), twice daily in a whole body smoke exposure system (SIU48, PROMECH LAB AB, Vintrie, Sweden). Mice were subsequently euthanized and the lungs inflated with a low-gelling agarose. 120 μm precision cut lung slices (PCLS) were generated. PCLS from room-air and cigarette smoke exposed mice were stimulated ex vivo with TLR agonists (Pam3CSK for TLR2, LPS for TLR4), as well as live and heat-killed versions of Hemophilus Influenza and S. Pneumoniae. Samples were collected 6h, 12h and 24h post-stimulation for gene expression profiling.
 
Contributor(s) Bauer CT, Golji J, Lambert K, Ravindran P, Cheng DT, Giron M, Allard J, Hilton H, Bitter H, Stampfli M, Stevenson CS
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Submission date Oct 18, 2012
Last update date Feb 11, 2019
Contact name Donavan Cheng
E-mail donavan.cheng@gmail.com
Organization name Hoffmann-LaRoche
Department Translational Research Sciences
Street address 340 Kingsland Ave
City Nutley
State/province NJ
ZIP/Postal code 07110
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (167)
GSM1022429 1001_RA_12_Heat-killed H. Flu RA_12_hkflu1_1001
GSM1022430 1002_RA_24_Live S. Pneumo RA_24_lpne1_1002
GSM1022431 1003_RA_12_Mock RA_12_mock1_1003
Relations
BioProject PRJNA177915

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41684_RAW.tar 608.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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