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Series GSE41358 Query DataSets for GSE41358
Status Public on Apr 05, 2013
Title Expression data from mouse preimplantation cloned embryos
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transcriptiome analysis is an excellent approach to understand the mechanism underlying nuclear reprogramming in somatic-cell-cloned embryos. Analysis of the transcriptomic data from the oocyte to blastocyst stage revealed that specific genes were inappropriately reprogrammed at each stage. Sertoli cell-cloned embryos appear to develop normally because the progression of incorrect reprogramming is concealed throughout development.
The time-lapse gene expression profiles provide valuable information for understanding reprogramming in SCNT embryos.
Overall design Single mouse oocyte (MII metaphase), in vitro fertilized embryo (replicates = 3 / each stage) and Sertoli cell cloned embryo (replicates = 5 / each stage) from 1-cell stage to blastocyst stage (16, 32, 48, 62, 72, 84 hours after sperm addition or activation) was used for total RNA extraction. Totally 51 Affymetrix microarrays were used in the experiment.
Contributor(s) Cao F, Watanabe H
Citation(s) 23580949
Submission date Oct 05, 2012
Last update date Feb 11, 2019
Contact name Feng Cao
Organization name Tokyo University of Agriculture
Department Bioscience
Street address 1-1-1 Sakuraoka, Setagaya-ku
City Tokyo
ZIP/Postal code 156-8502
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (51)
GSM1015455 Metaphase II oocyte, biological rep1
GSM1015456 Metaphase II oocyte, biological rep2
GSM1015457 Metaphase II oocyte, biological rep3
BioProject PRJNA176881

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Supplementary file Size Download File type/resource
GSE41358_RAW.tar 178.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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