|
| Status |
Public on May 19, 2013 |
| Title |
Single-cell transcriptomics reveals widespread heterogeneity in gene regulation and RNA processing in stimulated immune cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we used single-cell RNA-Seq to discover extensive cell-to-cell variability – in transcript expression levels, cell state, circuit usage, and alternative splicing – between stimulated immune cells
|
| |
|
| Overall design |
RNA seq libraries from 18 single cells, 3 populations of 10,000 cells, and 2 zero cells (negative controls) with SMARTer. Three additional single cell libraries with molecular barcodes (MB) using a modified SMARTer protocol.
|
| |
|
| Contributor(s) |
Shalek AK, Satija R, Park H, Regev A |
| Citation(s) |
23685454 |
| |
| Submission date |
Oct 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Rahul Satija |
| E-mail(s) |
rsatija@nygenome.org
|
| Phone |
6177022468
|
| Organization name |
New York Genome Center
|
| Lab |
Satija Lab
|
| Street address |
101 Avenue of the Americas
|
| City |
New York City |
| State/province |
NY |
| ZIP/Postal code |
10013 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA176414 |
| SRA |
SRP015959 |
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