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Series GSE41061 Query DataSets for GSE41061
Status Public on Aug 26, 2013
Title Methylation Changes in Soybean Mid-Maturation Seed Parts
Organism Glycine max
Experiment type Methylation profiling by high throughput sequencing
Summary Seeds are comprised of three majors parts of distinct parental origin: the seed coat, embryo, and endosperm. The maternally-derived seed coat is important for nurturing and protecting the seeds during development. By contrast, the embryo and the endosperm are derived from a double fertilization event, where one sperm fertilizes the egg to form the diploid zygote and the other sperm fertilizes the central cell to form the triploid endosperm. Each seed parts undergo distinct developmental programs during seed development. What methylation changes occurring in the different seed parts, if any, remains unknown. To uncover the possible role of DNA methylation in different parts of the seed, we characterized the methylome of three major parts of an early maturation stage seed: seed coat, embryonic cotyledons, and embryonic axis using Illumina sequencing.
 
Overall design Illumina sequencing of bisulfite-converted genomic DNA from three parts of an mid-maturation (B1) stage seed: seed coat (B1-SC), embryonic cotyledons (B1-COT), and embryonic axis (B1-AX).
 
Contributor(s) Goldberg RB, Chen M, Lin J, Le BH
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Submission date Sep 21, 2012
Last update date Dec 07, 2016
Contact name Bob Goldberg
E-mail bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL15008 Illumina HiSeq 2000 (Glycine max)
Samples (3)
GSM1008125 Mid-maturation-stage seed coat
GSM1008126 Mid-maturation-stage embryonic cotyledons
GSM1023628 Mid-maturation-stage embryonic axis
Relations
SRA SRP015838
BioProject PRJNA178372

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41061_RAW.tar 12.0 Gb (http)(custom) TAR (of TXT)
GSE41061_processed_readme.txt 672 b (ftp)(http) TXT
Raw data are available in SRA
Processed data provided as supplementary file

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