|
| Status |
Public on Aug 16, 2013 |
| Title |
Retinoic acid determines the in vivo fate-commitment of splenic pre-dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Comparison of gene expression changes of FACS sorted splenic CD11b+CD8a- and CD11b-CD8a+ cDC subsets reconstituted in vivo following total body irradiation in combination with exogenous retinoic acid or vehicle control.
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| |
|
| Overall design |
Mice maintained on a control diet were subjected to 6 Gy total body irradiation and then provided exogenous retinoic acid or vehicle control beginning on D(+)3 after irradiation. All treated mice also received adoptive T cell transfer of either Trp-1 CD4+ or Pmel-1 CD8+ T cells, rVV, and IL-2. Splenic cDCs were isolated on D(+)10 after total body irradiation by FACS sorting.
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| |
|
| Contributor(s) |
Klebanoff CA, Ferreyra GA |
| Citation(s) |
23999499 |
| Submission date |
Sep 20, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Gabriela A Ferreyra |
| Organization name |
NIH
|
| Department |
Critical Care Medicine Department
|
| Lab |
Functional Genomics and Proteomics Facility
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (16)
|
| GSM1006916 |
CD11b-CD8a+ with exogenous RA replicate 1 |
| GSM1006917 |
CD11b-CD8a+ with exogenous RA replicate 2 |
| GSM1006918 |
CD11b-CD8a+ with exogenous RA replicate 3 |
| GSM1006919 |
CD11b-CD8a+ with exogenous RA replicate 4 |
| GSM1006920 |
CD11b-CD8a+ with vehicle control replicate 1 |
| GSM1006921 |
CD11b-CD8a+ with vehicle control replicate 2 |
| GSM1006922 |
CD11b-CD8a+ with vehicle control replicate 3 |
| GSM1006923 |
CD11b-CD8a+ with vehicle control replicate 4 |
| GSM1006924 |
CD11b+CD8a- with exogenous RA replicate 1 |
| GSM1006925 |
CD11b+CD8a- with exogenous RA replicate 2 |
| GSM1006926 |
CD11b+CD8a- with exogenous RA replicate 3 |
| GSM1006927 |
CD11b+CD8a- with exogenous RA replicate 4 |
| GSM1006928 |
CD11b+CD8a- with vehicle control replicate 1 |
| GSM1006929 |
CD11b+CD8a- with vehicle control replicate 2 |
| GSM1006930 |
CD11b+CD8a- with vehicle control replicate 3 |
| GSM1006931 |
CD11b+CD8a- with vehicle control replicate 4 |
|
| Relations |
| BioProject |
PRJNA175555 |
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